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Selective media
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have been modified to suppress or prevent the growth of normal flora while permitting the growth of suspected pathogens
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Various Bacteria
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Selective media are designed to tell the difference between ? and to separate or select possible pathogens from a population of many different bacteria
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...
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The altering of the ? of the medium takes advantage of some organisms ability to grow in one ? and not another.
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Crystal violet
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The addition of a chemical such as ? retards bacterial growth of most gram positive bacteria without affecting the gram negative groups
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Antibiotics
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May be used for selective inhibition
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Enrichment
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Suppresses the growth of competitive normal flora while enhancing the growth of the desired organisms
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Normal flora
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Specimens known to be highly contaminated with ? often require exposure to enrichment media. (allows isolation of primary pathogen)
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Salmonella, shigella Agar (SS Agar)
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A highly selective medium for the isolation of Salmonella spp and shigella spp from fecal specimens
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Lactose and dyes
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In the SS agar ? are added to an agar base made from bile salts and beef extracts
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coliform (intestinal)
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SS Agar gives maximum inhibition of ? organisms without restricting pathogenic gram negative bacilli.
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Non-lactose fermenters
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Salmonella spp and shigella app are ? that form opaque or translucent (uncolored) colonies and are easily identified.
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Lactose fermenters
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will form a pink color in the colony
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Black centers
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Some Salmonella spp. may produce colonies with
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Gram Negative (GN) Broth
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A selective liquid medium used for the cultivation of gram-negative organisms from all types of specimens.
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Gram-negative
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Swabs containing suspected ? organisms should be inoculated into GN broth to increase the likelihood of isolating the organisms
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Sabouraud Dextrose Agar (SDA)
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Recommended for culturing yeasts and molds
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Pathogenic fungi
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Sabouraud Dextrose Agar (SDA) allows for the growth of most ? , antibiotics are often added to inhibit bacteria and nonpathogenic fungi
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Lowestein-Jensen Medium (LJ)
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Recommended for the isolation of Mycobacterium spp.
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malachite green
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Mycobacteria grow well while contaminants are inhibited by
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