question
tween hydrolysis
answer
distinguishes m scrofulceum from m gordonae
question
s aureus
answer
beta-lactamase inducible
question
combination antibiotic therapy
answer
synergistic effects should be assessed
question
typical of c fetus
answer
growth @ 37C
question
first 7-10 days of infection
answer
blood cultures from a case of suspected leptospiremia should be collected:
question
indole positive
answer
bacteroides thetaiotaomicron
question
H2S production
answer
what feature distinguishes e. rhusiopathiae from other clinically significant nonsupport forming. gram positive facultatively anaerobic bacilli
question
quenching agent
answer
potassium permanganate is used as what when using truant ermine-rhodamine stain
question
m. tuberculosis
answer
rough, non pigmented colony, flow growth @37C, niacin production positive, nitrate reduction positive
question
result of beta carotene formation and accumulation
answer
pigment production by m Kansasii
question
interfere w/cell wall synthesis
answer
microorganisms resembling l-forms have been treated with antibiotics that:
question
30%
answer
uv lamp should be replace when the intensity is what percentage compared to the original output
question
microsporum audouinii
answer
fluoresced yellow-green with Woods lamp, slow growing flat gray colony with salmon pink reverse.
microscopic- racquet hyphae, pectinate bodies, chlamydospores and a few abortive or bizarre shaped macroconidia.
microscopic- racquet hyphae, pectinate bodies, chlamydospores and a few abortive or bizarre shaped macroconidia.
question
sporothrix schenckii
answer
grown @25C: delicate septate hyaline hyphae and many condidophores at right angles. oval 2-5m condo formed @ end of conidiophores giving flowerlike appearance
grown 37C: small cigar shaped yeast cells
grown 37C: small cigar shaped yeast cells
question
e. floccosum
answer
smooth walls, club shaped
question
measles
answer
live attenuated vaccine
question
n sicca
answer
bap: pinpoint
choc: sm yellowish
gs:gnc
dextrose- acid
sucrose- acid
maltose- acid
lactose-neg
ox pos
beta-galactosidase neg
choc: sm yellowish
gs:gnc
dextrose- acid
sucrose- acid
maltose- acid
lactose-neg
ox pos
beta-galactosidase neg
question
cryptosporidium spp
answer
coccidian oocysts are inefective immediately after passage in the stool
question
m avium
answer
not included in the m tuberculosis complex
question
m xenopi
answer
which mycobacterium is often recovered from hot water systems, causing nosocomial infections in hospitals via water storage taanks
question
all use 5% sheep blood agar
answer
isolation and growth staph and strep
determining hemolytic patterns
CAMP test for B strep
determining hemolytic patterns
CAMP test for B strep
question
decolorizer reagents
answer
modified acid fast staining differs from Ziehl-neelsen staining in the:
question
e coli
answer
gnb
lactose pos
indole pos
lactose pos
indole pos
question
routine ova and parasite exam
answer
fecal immunoassays alternative to this
question
x factor requirement for growth
answer
porphyrin test (ALA) is a confirmatory procedure for which test of haemophilus spp
question
strength of destaining reagents
answer
main difference between ziehl-neelsen stain and a modified acid fast stain is:
question
m leprae
answer
which mycobacterium app cannot be cultivated on media
question
CCFA
answer
best agar to recover C dif from a patient who has antibiotic assoc. diarrhea
question
p. aeruginosa
answer
organism most often isolated from pulmonary secretions of cystic fibrosis patients
question
stain strength and staining time
answer
difference between trichrome and modified trichrome stains include
question
sorbitol
answer
e coli isolate recovered from pt w/severe bloody diarrhea. what sugar should be tested for before serotyping
question
Factor V only
answer
H parainfluenza requires which factor(s) for growth
question
afb stains
answer
carbol-fuchsin and methylene blue
question
cryptosporidium spp
answer
fecal smear shows pink stained oocysts w/modified ziehl-neelsen acid fast staining tecniqu
question
eosinophilia
answer
significant following infection with visceral larva migrans
question
cytochrome oxidase
answer
helps to differentiate b. cepacia from s. maltophilia
question
clumping factor
answer
slide coagulase test (rabbit plasma) detects what coat factor
question
young children
answer
accidental ingestion of raccoon roundworm eggs has led to severe disease in:
question
oxidase, motility and NO3 reduction
answer
tests to differentiate a. baumanii from p. aeruginosa
question
zygomycetes group
answer
mucor, absidia, rhizopus
question
breakpoint
answer
level of drug that is needed for optimal therapy in vivo
level of drug achievable in serum by MIC method
level of drug achievable in serum by MIC method
question
b fragilis
answer
gnb (pleomorphic)
anaerobe
bile esculin- pos
resistant to kanamycin, vanco, and penicillin
anaerobe
bile esculin- pos
resistant to kanamycin, vanco, and penicillin
question
isospora belli
answer
these oocysts are normally seen in the concentration sediment wet mount
question
a. hydrophilia
answer
use a cytochrome oxidase test to differentiate this from normal fecal flora of enterobacteriaceae family
question
c difficile
answer
scant normal fecal flora from nursing home pt
CCFA grew large yellpw rhizoid colonies. what anaerobe?
CCFA grew large yellpw rhizoid colonies. what anaerobe?
question
group A strep
answer
bacitracin disks are used to identify what organism
question
100x
answer
what objective is used to view microsporidian spores in human specimens
question
neg DNase
answer
cedecea are differentiated from serrate by what test result
question
gram stain, motility catalase
answer
tests used to differentiate between listeria and strep
question
the polar tubule
answer
horizontal strip that is described w/in the microsporidial spore is
question
pneumonia,sepsis and meningitis
answer
assoc. w/disseminated strongyloidiasis
question
ONPG test
answer
detects b galactosidase- positive activity in e coli
helps differentiate shigella from e coli
helps differentiate shigella from e coli
question
2% NaOH and N-acetyl-L-cysteine
answer
what sputum specimens for AFB must be digested and decontaminated with
question
ox pos gnb
answer
a. hydrophilia
plesiomonas spp
v. cholerae
plesiomonas spp
v. cholerae
question
pos for nitrate reduction
answer
red color after addition of sulfanilic acid and alpha-naphthylamine means
question
predominance of neutrophils
answer
suitable sputum smear should show
question
b fragilis
answer
anaerobic GNb from gall bladder
kanamycin and vaco resistant
growth on 20%bile
kanamycin and vaco resistant
growth on 20%bile
question
modified trichrome stain
answer
used to identify microsporidian spores
question
hansens bacillus
answer
m. leprae
question
x factor
answer
porphyrin test (ALA) is confirmatory procedure for which test (haemophilus spp)
question
coccidioides immitis
answer
mycelial form of which dimorphic mold produces a think walled rectangular or barrel shaped alternat arthroconidia
question
disseminated strongyloidiasis
answer
autoinfective feature of life cycle
question
vibrio
answer
what uses TCS (thiosulfate citrate bile sucrose) agar
question
internal contents, infectivity and size
answer
these differentiate c. cayetanensis from cryptosporidium
question
a baumanii
answer
gnb, ox neg non motile
question
h flu
answer
this causes purulent conjunctivitis
question
antibiotics and nutrient
answer
specimens for viral culture should be transported in media containing
question
acetoin
answer
the VP test detects what end product of glucose fermentation
question
salmonella spp
answer
lysine iron agar (LIA) showing purple slant and blackened butt indicates
question
morganella, providencia and proteus
answer
positive for phenylalanine deaminsae
question
s sonnei
answer
most commonly assoc with diarrheal disease in US
question
p vulgaris
answer
ornithine neg
question
p mirabilis
answer
ornithine pos
question
cetrimide agar
answer
this agar is used as a selective isolation agar for p aeruginosa
question
b
answer
majority h. flu infections are caused by which serotypes
question
assoc w/ endocarditis
answer
h. aphrophilus
question
h. ducreyi
answer
which homophiles spp is difficult to isolate and recover from genital and ulcers and swollen lymph nodes
question
oxalic acid
answer
agent used for processing specimens for mycobacterial culture contaminated w/Pseudomonas
question
rotavirus
answer
only detected by electron scope or EIA methods
question
penicillin may inhibit some strains of B pertussis
answer
why has cephalexin been recommended to be used in bordet-gengou agar instead of penicillin
question
oxidase positive
answer
legionella pneumophilia is
question
DNA probe
answer
differentiation of M avium from M intracellular can be accomplished by
question
aspirate urine aseptically from catheter tubing
answer
most appropriate method for collecting urine from pt with indwelling cath
question
penicillin derivative and aminoglycoside
answer
what 2 different antimicrobial agents are commonly used , poss synergistic action, to treat endocarditis caused by e faecalis
question
reason to use 2 or more drugs for pulmonary tuberculosis
answer
prevent the emergence od bacilli resistant to the action of either or both drugs
question
capacity to withstand intracellular digestion by macrophages
answer
disease producing capacity of m tuberculosis depend upon
question
m intracellular-avium
answer
most likeyl assoc. with resistance to standard drug therapy
question
m tuberculosis
answer
used as pos control for nitrate test for mycobacteria with a reagent impregnated paper strip
question
endothelial
answer
rickettsiae infecting man multiply preferentially within which cells
question
lack cell walls
answer
mycoplasmas differ from bacteria b/c they:
question
complement fixation test
answer
most reliable serologic test for diagnosis of Q fever is
question
urine
answer
preferred specimen for lab diagnosis of acquired CMV infection
question
excess heating in preparation of the medium
answer
batch of choc agar grew h flu only as satellite colonies around s aureus. why?
question
virus rarely recoverable from CSF or feces during herpes encephalitis
answer
diagnosis of herpes simplex encephalitis caused by herpes simplex 1
question
E coli, p vulgaris
answer
imvic= ++--
question
shigella spp
answer
imvic= -+--
question
salmonella, p mirabilis, c freundii
answer
imvic= -+-+
question
klebsiella, e. aerongenes
answer
imvic= --++
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