question
STAPHYLOCOCCUS & MICROCOCCUS are catalase
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Positive
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STAPHYLOCOCCUS & MICROCOCCUS are Normal inhabitants of the
answer
skin and mucous membranes
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STAPHYLOCOCCUS & MICROCOCCUS type of bacteria
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Gram-positive cocci, in clusters
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Staphylococcus coagulase
answer
varlable
question
Micrococcus Coagulase
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negative
question
Staphylococcus OF glucose
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fermenter
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Micrococcus OF glucose
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oxidizer
question
Staphylococcus Bacitracin
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resistant
question
Micrococcus Bacitracin
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susceptible
question
Staphylococcus Lysostaphin
answer
susceptible
question
Micrococcus Lysostaphin
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resistant
question
Staphylococcus Modified Oxidase
answer
negative
question
Micrococcus Modified Oxidase
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positive
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Staphylococcus aureus virulence factor that is associated with food poisoning (most frequently A, B, and D)
- resistant to gastric juice
heat stable
- resistant to gastric juice
heat stable
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enterotoxins A
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Staphylococcus aureus virulence factor that is produced by S. aureus phage group I
- causes toxic shock syndrome (TSS)
- causes toxic shock syndrome (TSS)
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enterotoxin F /exotoxin C/TSST-1
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Staphylococcus aureus virulence factor that is produced by S. aureus phage group Il
- causes scalded skin syndrome (SSS)
- causes scalded skin syndrome (SSS)
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exfoliative toxin/epidermalytic toxin
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Staphylococcus aureus virulence factor that binds the Fc portion of the lg and aids the bacterium avoid phagocytosis by interfering with opsonization
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Protein A
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Have hyaluronidase(spreading factor)
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Staphylococcus aureus
question
What kind of virulence factor is Panton-Valentine leukocidin
answer
cytolytic toxins
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hospital outbreaks may occur in infant nurseries, burn units and in those who have undergone surgery (Nosocomial outbreaks)
answer
Staphylococcus aureus
question
inhabits the anterior nares and perineum
answer
Staphylococcus aureus
question
transmission is by direct contact with contaminated hands or by fomites
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Staphylococcus aureus
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A clinical infection of S. aureus with lesions of folliculitis may develop into a deeper inflammatory nodule called a
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boils/furuncle
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A clinical infection of S. aureus with abscess that extends even more deeply into the subcutaneous fat and may have multiple draining sites
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carbuncle
question
A clinical infection of S. aureus with pustules are larger and surrounded by a small zone of erythema compared to strep impetigo; highly contagious
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impetigo
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A clinical infection of S. aureus with multisystem disease characterized by high fever, rash, hypotension, and shock
- associated with tampon use but may also be seen in males and non-menstruating
females
- associated with tampon use but may also be seen in males and non-menstruating
females
answer
TSS (toxic shock syndrome)
question
A clinical infection of S. aureus more common in newborns and adults with chronic renal failure and impaired immune system (the condition may be confused with Lyell's syndrome/toxic epidermal necrolysis)
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Scalded Skin Syndrome(SSS) / Ritter disease
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Nosocomial Infections in S. Aureus :
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bacteremia, endocarditis, pneumonia
question
In S. aureus, what is seen in prepubertal children
answer
Septic arthritis
question
most common cause of hospital-acquired UTI
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Staphylococcus epidermidis
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slime enables the organism to adhere to the surface of prosthetic devices
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Staphylococcus epidermidis
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prosthetic valve endocarditis and immunocompromised patients are most susceptible in
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Staphylococcus epidermidis
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associated with UTI among young sexually active females
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Staphylococcus saprophyticus
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adheres to the urogenital tract; rarely found in other skin areas and mucous membranes
answer
Staphylococcus saprophyticus
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Microscopic exam appears like Bunches of grapes
answer
Staphylococcus saprophyticus
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Staphylococcus saprophyticus color of colonies in Blood Agar
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cream or off white
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S. aureus may demonstrate ______ hemolysis
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beta
question
selective and differential medium useful in recovery and identification of Staphylococcus aureus from specimens with mixed flora
answer
Mannitol salt agar (MSA)
question
mannitol fermentation produces acid products and the lowered pH changes the color of______ to _______
answer
phenol red to yellow
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In MSA, colonies of S. aureus appear
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yellow
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In MSA, S. epidermidis colonies appear
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reddish
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may be able to grow on MS and produce slight mannitol fermentation but is catalase negative
answer
Enterococcus
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selective enrichment medium for isolation of g(+) cocci
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Columbia colistin-nalidixic acid agar (CNA)
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colistin and nalidixic acid + __________ for enrichment
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sheep's blood
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colistin and nalidixic acid inhibit
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g- organisms
question
selective medium to isolate g(+) cocci from specimens with mixed flora
answer
Phenylethyl alcohol agar (PEA)
question
inhibits facultative g(-) bacilli (esp swarming Proteus) but
permits the growth of g(+) cocci
permits the growth of g(+) cocci
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phenylethyl alcohol
question
Test used to differentiate staphylococci from streptococci
answer
catalase test
question
Positive result of catalase test
answer
Rapid bubbles
question
Used to differentiate Micrococcus from coag(-]staphylucoccuS
answer
Modified Oxidase (Microdase)
question
Modified Oxidase (Microdase) positive reaction:
answer
purple/blue in 30 secs
question
used to differentiate S. aureus from coag-negative staph
answer
Coagulase
question
major marker for S. aureus;
answer
coagulase clots plasma
question
Detects Cell Bound coagulase clumping factor
answer
slide coagulase Test
question
slide coagulase Test positive result
answer
clumping
question
detects clumping factor and protein A in the cell wall of S, aureus
answer
latex agglutination
question
The best test for staphaurex
answer
latex agglutination
question
Detects extracellular free coagulase
answer
tube coagulase test
question
do not produce cell-bound coagulase
answer
5% of S. aureus
question
negative slide coagulase test result must be confirmed with the
answer
tube method
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