question
What is in the air we breath
answer
-21% 02
-78% N2
-1% Argon
-<0.17% CO2
-78% N2
-1% Argon
-<0.17% CO2
question
Obligate Aerobe bacetria
answer
-Require O2
*EX: Micrococcus, Bacillus
*EX: Micrococcus, Bacillus
question
Obligate anaerobic bacteria
answer
-Dies when expose to O2
question
Microaerophilic bacteria
answer
-Tolerate a small amount of O2
*EX: Campy, Borrelia
*EX: Campy, Borrelia
question
Facultative anaerobic bacteria
answer
-Grow with or without oxygen
-Facultative + Oxidative
*EX: Streps, and Staphs
-Facultative + Oxidative
*EX: Streps, and Staphs
question
Aerotolerant anaerobe
answer
-Microorganism unable to respire O2 but whose growth is unaffected by it
question
Capnophile
answer
-Require high CO2 to grow
*EX: Haemophilus, Nissera
*EX: Haemophilus, Nissera
question
Where are Anaerobes found as normal flora
answer
-Oral cavity
-GI tract
-Genitourinary tract
-Skin
-GI tract
-Genitourinary tract
-Skin
question
Fusobacterium
answer
-GNB anaerobe
-Normal flora: Upper RT, and GI tract
-Normal flora: Upper RT, and GI tract
question
Fusobacterium nucleatum causes
answer
-Severe pulmonary infection
question
Fusobacterium necrophorum causes
answer
-Lemievve syndrome
-Neck pain + Throat infection
-Neck pain + Throat infection
question
Porphyromonas is a
answer
-GNB anaerobe
-Normal flora: Oropharynx, GI tract, Urogenital tract
-Normal flora: Oropharynx, GI tract, Urogenital tract
question
Porphyromonas identification
answer
-Brick red colonies under UV light
-Doesn't grow in 20% Bile
-Vanc (S)
-Kanamycin (R)
-Doesn't grow in 20% Bile
-Vanc (S)
-Kanamycin (R)
question
What organism causes periodontitis
answer
Porphyromonas gingivalis
question
Prevotella melaninogenica
answer
-GNB anaerobe
-Normal flora: Oropharynx, Nose, GI tract, Urogenital tract
*Bite wounds
-Normal flora: Oropharynx, Nose, GI tract, Urogenital tract
*Bite wounds
question
Prevotella melaninogenica identification
answer
-Brick Red colonies under UV light
-Doesn't grow in 20% Bile
-Vanc (R)
-Kanamycin (R)
-Doesn't grow in 20% Bile
-Vanc (R)
-Kanamycin (R)
question
Bacterodies fragilis
answer
-GNB anaerobe
-Normal flora: Colon
*Major cause of anaerobic infection (Post surgery)
-Normal flora: Colon
*Major cause of anaerobic infection (Post surgery)
question
Bacterodies fragilis identification
answer
-Non pigmented colonies
-GROWS in 20% Bile
-Penicillin, Kanamycin, Vanc (R)
-Rifampin (S)
-GROWS in 20% Bile
-Penicillin, Kanamycin, Vanc (R)
-Rifampin (S)
question
Clostridium perfringens
answer
-GPB spore forming
-Normal flora: GI tract
-Normal flora: GI tract
question
Clostridium perfringens identification
answer
-Double zone of hemolysis
-GP Box car cell morphology
-Lecithinase (+)
-Best way to see spores---> Direct Gram stain
-GP Box car cell morphology
-Lecithinase (+)
-Best way to see spores---> Direct Gram stain
question
Clostridium perfringens diseaes
answer
-Gas gangrene
-Enterocolitis
-Food poisoning
-Enterocolitis
-Food poisoning
question
Clostridium perfringens effects
answer
-Diabetics
-PPL with circulatory disease
-PPL with circulatory disease
question
Clostridium tetani
answer
-GPB spore-forming
-Causes lockjaw-----> Rigid paralysis
-Toxin released
-Prevention: Dtap vax
-Causes lockjaw-----> Rigid paralysis
-Toxin released
-Prevention: Dtap vax
question
Clostridium botulinum
answer
-GPB spore forming
-Causes botulism----> Flaccid paralysis
-Acquired from spoiled canned goods
-Infant botulism from honey
*Botox
-Causes botulism----> Flaccid paralysis
-Acquired from spoiled canned goods
-Infant botulism from honey
*Botox
question
Clostridium difficile
answer
-GPB spore forming
-Normal flora: GI tract
-Prominent in hospitals
-Normal flora: GI tract
-Prominent in hospitals
question
How does Clostridium difficile cause disease
answer
-Antibiotics kills normal flora---> increase of C. diff
-Toxin released----> Pseudomembranous
-Hand washing kills spores
-Toxin released----> Pseudomembranous
-Hand washing kills spores
question
30% Clostridium difficile is not pathogenic
answer
-Cell culture and look for cytopathic effect= pathogenic
-PCR or Toxin-EIA (+) = pathogenic
-PCR or Toxin-EIA (+) = pathogenic
question
Lactobacillus
answer
-GPB
-Normal flora of vaginal tract
-Catalase (+)
-Rapid growth rate
-Looping chains
-Normal flora of vaginal tract
-Catalase (+)
-Rapid growth rate
-Looping chains
question
Cutibacterium (Propionibacterium)
answer
-GPB
-Catalase (+)
-Moderate growth rate
-Anerobic diptheroid
-Catalase (+)
-Moderate growth rate
-Anerobic diptheroid
question
Actonomyces
answer
-GPB
-Catalase (-)
-Slow growth rate
-Filamentous/ branching gram stain
-Catalase (-)
-Slow growth rate
-Filamentous/ branching gram stain
question
Diseases: Exogenous source
answer
-Toxins in soil or toxins
*Botulism
*Gas ganrene
*Tetanus
*Botulism
*Gas ganrene
*Tetanus
question
Diseases: Endogenous source
answer
-Normal flora----> Clinically significant
*Actinomycosis
*Aspiration pneumonia
*Necrotizing pneumonia
*Peridontal
*Actinomycosis
*Aspiration pneumonia
*Necrotizing pneumonia
*Peridontal
question
Atopobium that cause bacterial vaginosis
answer
*Atopobium vaginae
*Gardnella
*Mobilunaus
*Mycoplasma
*Ureaplasma
*Gardnella
*Mobilunaus
*Mycoplasma
*Ureaplasma
question
Good specimens to grow anaerobic organsima
answer
-Specimens not exposed to O2
-Deep specimens
-Deep specimens
question
Aerotolerance Test
answer
-Determines whether a microorganism is a strict anaerobe or a facultative anaerobe
-Use blood agar and CDC agar
-Use blood agar and CDC agar
question
Organism that grows in Blood agar (R) and CDC agar (N)
answer
-Facultative
-R: Aerobic
-N: Anaerobic
-R: Aerobic
-N: Anaerobic
question
Organism that don't grow on Blood agar (R) but does on CDC agar (N)
answer
-Strict anaerob
question
CDC agar
answer
-Anaerobic blood
question
PEA and KV agar
answer
-Selective agar
question
BBE agar
answer
-Bacteroides/ Bile esculin
-Selective + Differential
-Selective + Differential
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