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metabolism
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chemical processes that sustain life of organisms
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endoenzyme
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produced and function inside the cell
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exoenzyme
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produce inside the cell, but function outside the cell; break down large molecules
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selective media
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contains chemicals that enhance and/or inhibit growth
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differential media
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distinguishes bacterium by how that metabolize or change the media with a waste product
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enriched media
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contains ingredients that enhance growth of desired bacteria, but allow others to grow as well
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acid metabolic mechanisms
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simple sugar -> endoenzyme -> acid
complex sugar -> exoenzyme -> simple sugar -> acid
complex sugar -> exoenzyme -> simple sugar -> acid
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ammonia metabolic mechanisms
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amino acid -> endoenzyme -> ammonia
protein -> exoenzyme -> amino acid -> ammonia
protein -> exoenzyme -> amino acid -> ammonia
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Eosin Methylene Blue (EMB) Agar
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selective for gram-negative bacteria and differential for organisms that ferment lactose; selective due to methylene blue and eosin; fermenters lower pH which interacts with eosin; non-fermenters increase pH by deamination of proteins
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Phenylethyl Alcohol Agar (PEA)
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selective for gram-positive bacteria; interferes with gram-neg DNA synthesis
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Thick, strong growth (EMB)
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gram negative
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Weak growth/no growth (EMB)
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gram positive
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pink, black or metallic green growth (EMB)
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lactose fermentation
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colorless growth (EMB)
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no lactose fermentation
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opaque growth (PEA)
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gram positive
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transparent growth/ weak growth/ no growth (PEA)
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gram negative