question
Columbia CNA with 5% sheep blood agar is
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undefined, differential, selctive medium
allows growth of gram + organisms ( especially staphylococci, streptococci, and enterococci) it inhibits growth of most gram negative organisms
allows growth of gram + organisms ( especially staphylococci, streptococci, and enterococci) it inhibits growth of most gram negative organisms
question
columbia agar
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casein, digest of animal tissue, beef extract, yeast extract, corn starch, and sheeps blood
question
what does sheeps blood provide
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the x factor(heme)
question
what does yeast extract provide
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B-vitamins
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the antibiotics in the medium are...
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colistin and nalidixic acid
question
the antibiotics act as selective agents against gram-negative organisms by affecting membrane integrity and interfering with
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DNA replication
-they are effective against Klebsiella, Proteus and Pseudomonas species
-they are effective against Klebsiella, Proteus and Pseudomonas species
question
sheep blood makes possible differentiation of gram positive organisms based on
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hemolytic reaction
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Columbia CNA agar is used to isolate staphylococci, streptococci, enterococci, primarily from
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clinical specimens
question
Columbia CNA agar with 5% sheep blood
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serves as a comparison for growth quality on the Columbia CNA with 5% sheep blood agar plate
question
what is the role of the nutrient agar
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organism is inhibited by colistin and nalidixic acid
probable gram-negative organism
probable gram-negative organism
question
poor growth(P) or no growth(N)
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organism is not inhibited by colistin and nalidixic acid and completely hemolyzes RBCs
probable beta-hemolytic
(staphylococcus, Streptococcus)
There might be artifactual greening with some beta hemolytic organisms
probable beta-hemolytic
(staphylococcus, Streptococcus)
There might be artifactual greening with some beta hemolytic organisms
question
good growth(G) with clearing of medium (beta-hemolysis)
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organism is not inhibited by colistin and nalidixic acid and partically hemolyzes RBCS
probable-alpha hemolytic (Staphylococcus, streptococcus)
probable-alpha hemolytic (Staphylococcus, streptococcus)
question
Goog growth(G) with greening of medium(alpha-hemolysis)
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organism is not inhibited by colistin and nalidixic acid and does not hemolyze RBCs
probable y-hemolytic (staphylococcus)
probable y-hemolytic (staphylococcus)
question
good growth (G) with no change of medium's color
answer
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