question
name a few concentration techniques for parasite diagnosis.
answer
filtration/ centrifugation, flotation/ sedimentation, Baermann test
question
what do fecal flotation tests detect?
answer
parasite eggs, larvae, cysts from the GI tract, liver and bile ducts
question
define the Baermann technique
answer
extracting nematode larvae from fresh feces by placing it in warm water, the larvae swims away from the feces and sinks to the bottom
question
what is a simple stain?
answer
staining technique using only 1 dye
question
what is a differential stain?
answer
staining technique using more than one dye
question
define viral inclusion bodies
answer
nuclear or cytoplasmic aggregates made of proteins that show presence of viral replication
question
what is the Romanowsky stain?
answer
diff-quick stain; uses a blue, red, and sapphire colored dye
question
what type of stain is gram-stain?
answer
differential stain
question
what type of stain is used for mycobacterium?
answer
acid fast stain
question
what is periodic acid Schiff?
answer
PAS; fungal stain that stains mucin
question
what is AMR?
answer
antimicrobial resistance
question
what are fastidious bacteria?
answer
bacteria that require specific growth factors and specific nutrients
question
why are mesophiles important?
answer
because they reproduce and thrive at temperatures similar to body temperatures
question
define unculturable
answer
bacteria that are not able to grow on artificial media
question
what does MacConkey agar detect?
answer
gram-negative and lactose fermentation (pink)
question
what does mannitol salt agar detect?
answer
gram-positive, mannitol fermentation (yellow)
question
what does CLED agar do?
answer
cysteine-lactose-electrolyte deficient; supports urinary pathogen growth, detect lactose fermentation
question
give an example of a biochemical test for bacterial identification
answer
UTI culture paddles
question
what is the flexicult vet urinary test?
answer
test which antibiotic would be more effective to existing urinary bacteria