Beta (total), alpha (partial), gamma (none)
Strep
Neisseria (if incubated in CO2)
Staph
Strep
E. coli
Klebsiella
Haemophilus
Neisseria
Strep
Salmonella
Shigella
e.g. Haemophilus, Neisseria, Campylobacter
Will respire in O2
Will ferment in CO2
Most pathogens
e.g. Haemophilus, Staphylococcus, E. coli, Yersinia pestis
e.g. Mycobacterium tuberculosis, Pseudomonas aeruginosa
e.g. Actinomyces, Clostridium, Fusobacterium
e.g. Campylobacter
e.g. Campylobacter, Heliobacter pylori
Grown on Bordet-Gengou blood agar
Grown on Thayer-Martin or modified Thayer-Martin
-BSL-IV organism
-significant pathogen
-Streptococcua a galactose or herpes simplex virus from gential site of pregnant woman at term
Bordetella pertussis
Brucella
Legionella
Streptococcua pyogenes
All of the following are true of CBA except
a. contains nutrients for growth of fastidious bacteria
b. is a differential medium
c. supports the growth of Haemophilus
d. supports the growth of Neisseria gonorrhoeae
Which of the following is true of SBA?
a. supports the growth of Haemophilus
b. supports the growth of Neisseria gonorrhoeae
c. colonies of beta-hemolytic streptococci will demonstrate clear zones on this medium
d. is a selective, differential medium
All of the following are true of CNA except
a. supports the growth of staphylococci
b. supports the growth of E. coli
c. supports the growth of streptococci
d. is a selective agar
Which of the following will grow on EMB?
a. Staphylococcus aureus
b. Group B streptococcus
c. Haemophilus influenzae
d. Klebsiella pneumoniae
All of the following is true of EMB except
a. is a selective, differential medium
b. inhibits growth of Gram positive cocci
c. lactose fermenters grow as clear-pink colonies
d. lactose fermenters grow as dark-blue colonies
Which of the following media is used to isolate Vibrio species from stool specimens?
a. EMB
b. TCBS
c. HE
d. CIN
All of the following are true of HE except
a. selects for growth of enteric pathogens from feces
b. detects H2S production
c. Salmonella and Shigella produce blue-green colonies
d. The differential ingredients include glucose, lactose, and sucrose
What organism does CIN select for?
a. Campylobacter
b. Yersinia enterocolitica
c. Salmonella
d. Vibrio cholerae
Which of the following agars is used for isolation of Bordetella pertussis?
a. charcoal agar
b. BCYE agar
c. C5 agar
d. New York City medium
Proper media culture of a urethral discharge from a man include
a. sheep blood and polyethyl alcohol agars
b. EMB and SBA
c. thioglycollate broth and CBA
d. CBA and MTM
d. CBA and MTM
for the recovery of Neisseria gonorrhoeae
Which of the following clean catch urine culture colony counts indicates a patient likely has a urinary tract infection.
a. 10^1 CFU/mL
b. 10^3 CFU/mL
c. 10^5 CFU/mL
d. no growth
Which selective medium is used for isolation of Gram positive organisms?
a. CNA
b. trypticase soy agar with 5% sheep blood
c. EMB
d. MTM
a. CNA
Which of the following is the most appropriate specimen source and primary media battery?
a. endocervical: CBA & Martin-Lewis agar
b. sputum: SBA, TM, KV-laked blood agar
c. CSF: CNA & MAC
d. urine: SBA, CBA, CNA
Which of the following organisms must be incubated in a microaerophilic environment for optimal recovery of the organism?
a. Campylobacter jejuni
b. Escherichia coli
c. Pseudomonas aeruginosa
d. Proteus mirabilis
Chocolate agar base containing vancomycin, colistin, anisomycin, and trimethoprim is also known as
a. EMB
b. MTM
c. CNA
d. Martin-Lewis agar
d. Martin-Lewis agar
It's a modification of modified Thayer-Martin (MTM)
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