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Halotolerant (Osmotolerant)
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survive in 7.5%-10% NaCl
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staph aureus
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halotolerant example
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halotolerant survival
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survives by producing solutes when grown in high salt concentration. makes internal cytoplasm hypertonic to the outer environment
Proline, choline, betaine
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extreme halophiles
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require high salt concentrations to stabilize the cell wall and enzymes
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halobacterium salinarium
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extreme halophile examples
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extreme halophile survival
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transport K+ from external environment into cells and accumulate in large quantities, water then enters the cell to prevent dehydration
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glucose and fructose
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streptococci and lactobacilli produce acids from
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calcium
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as pH drops below 4.8, lactobacilli multiply and produce enough acid to remove
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Streptococcus, Lactobacillus
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__________ is mainly responsible for cavities, but the Snyder test measures acid production from _________
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obligate acidophiles
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only grow at pH of 2-3
hydrogen ions stabilize the cell membrane
hydrogen ions stabilize the cell membrane
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neutrophiles
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grow well in pH 6-9
most organisms
most organisms
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alkalophiles
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grow at pH 10-12
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transient flora
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commonly found on skin, not permanent, easily removed by normal handwashing
30 sec scrub removes most
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resident flora
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also called normal flora, live on and in your body. more permanent, harder to remove. 2 min scrub removes some
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pathogens in raw milk
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salmonella typhi
mycobacterium tuberculosis
brucella melitensis
e coli ( and other coliforms)
Coxiella burnetti
listeria monocytogenes
mycobacterium tuberculosis
brucella melitensis
e coli ( and other coliforms)
Coxiella burnetti
listeria monocytogenes
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Grade A standards
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no more than 20,000 bacteria/ mL. no more than 10 coliforms/ mL
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fermentation
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anaerobic alternative to cellular respiration
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homolactic
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microbes that produce only lactic acid
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heterolactic
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organisms that produce lactic acid as well as ethanol and CO2
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lactose fermentation test
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uses phenol red to measure the production of acidic end products
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yellow (phenol red)
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fermenter, indicates acid production
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red (phenol red)
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non-fermenter
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antimicrobials
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chemicals that kill microbes or stop their growth
phenols
halogens
alcohols
quats
phenols
halogens
alcohols
quats
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antibiotics
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antimicrobial substances produced by living organisms that kill or inhibit other microbes
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broad spectrum
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ability of a drug to be effective against a wide range of microorganisms G+ and G-
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narrow spectrum
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effective against specific bacteria
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zone of inhibition
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Region around a chemical saturated disc, where bacteria are unable to grow due to adverse effects of the compound in the disc.
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260 nm
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best UV radiation wavelength
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purine and pyrimidine bases
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UV is absorbed by
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pyrimidine dimers
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extra bonds formed between thymine or cytosine in DNA (cause of mutations) due to UV radiation
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photolyase
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in the presence of a longer wavelength repair dimerized bases
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strep throat
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acquire sample of the back of throat and tonsils
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strep pyogenes
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strep is caused by
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B hemolytic, G+ cocci
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strep pyogenes
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abscesses
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some naturally carry pathogenic strains. may or may not show signs of illness. can infect others and contaminate foos. usually carried in nares (can be other places)
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alpha hemolysis
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partial hemolysis (green)
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beta hemolysis
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complete hemolysis (clear)
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gamma hemolysis
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no hemolysis
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sheep blood agar
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hemolysis is observed on
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molds
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multicellular eukaryotes
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yeast
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unicellular eukaryotes
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Chlamydoconidia
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round, thick-walled
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blastoconidia
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budding off another spore
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arthroconidia
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rectangular, large
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zygomycetes
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aseptate hyphae, sporangiospores, zygospores
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ascomycetes
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septate hyphae, spores in ascus
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basidiomycetes
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dikaryon fusion hyphae, produce fruit (mushrooms)
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deuteromycetes
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asexual reproduction, human pathogens
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dimorphism
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mold at 25 C and yeast at 37 C