question
negative, none, no, chocolate, s. aureus
answer
haemophilus influenzae:
gram ____ coccobacilli
what type of hemolysis?
will it grow on SBA?
grows on ______ agar
growth factors X (hemin) and V (NAD)
______ can be seen if it is cultured with _____ on SBA because the RBC's will already be lysed
gram ____ coccobacilli
what type of hemolysis?
will it grow on SBA?
grows on ______ agar
growth factors X (hemin) and V (NAD)
______ can be seen if it is cultured with _____ on SBA because the RBC's will already be lysed
question
no, beta lactamase, polyribosyl ribitol phosphate (PRP), factor X/hemin and V/NAD
answer
haemophilus influenzae type B:
invasive
vaccine preventable
exotoxin production?
30% produce ______
more virulent than other types. young children at risk
has a capsule composed of __________ which inhibits bactericidal activity and interferes with phagocytosis
requires what growth factor(s)?
invasive
vaccine preventable
exotoxin production?
30% produce ______
more virulent than other types. young children at risk
has a capsule composed of __________ which inhibits bactericidal activity and interferes with phagocytosis
requires what growth factor(s)?
question
capsules are weak vaccine antigens
answer
what do you use conjugated vaccines for h. influenzae?
question
PCV13, Hib
answer
conjugate vaccines combine a strong antigen with a weak antigen.
______- polysaccharide from 13 serotypes conjugated to a nontoxic mutant of diphtheria toxin and an aluminum adjuvant
_______- PRP conjugated with either tetanus toxoid or other membrane protein complex of Neisseria meningitidis
______- polysaccharide from 13 serotypes conjugated to a nontoxic mutant of diphtheria toxin and an aluminum adjuvant
_______- PRP conjugated with either tetanus toxoid or other membrane protein complex of Neisseria meningitidis
question
bordetella pertussis
answer
what organism?
gram negative coccobacillus
strict aerobe
cause of whooping cough
humans are only reservoir
attach to ciliated epithelium of respiratory tract
gram negative coccobacillus
strict aerobe
cause of whooping cough
humans are only reservoir
attach to ciliated epithelium of respiratory tract
question
pertactin, hemagglutinin, cAMP
answer
bordetella pertussis:
virulence factors facilitate attachment to cilia: _____ and _____
pertussis toxin increases ____ levels and inhibits phagocytosis
virulence factors facilitate attachment to cilia: _____ and _____
pertussis toxin increases ____ levels and inhibits phagocytosis
question
catarrhal, paroxysmal, convalescent
answer
whooping cough:
caused by bordetella pertussis
______ stage: serious rhinorrhea, sneezing, fever. very infectious
______ stage: 1-2 weeks later. mucus clearance impaired. whooping cough
_______ stage: 2-4 weeks later. cough diminishes
caused by bordetella pertussis
______ stage: serious rhinorrhea, sneezing, fever. very infectious
______ stage: 1-2 weeks later. mucus clearance impaired. whooping cough
_______ stage: 2-4 weeks later. cough diminishes
question
charcoal
answer
to grow bordetella, media containing ______ adsrobs toxins and facilitates growth
question
moraxella catarrhalis
answer
what organism?
gram negative diplococci
causes otitis, sinusitis, bronchitis, bronchopneumonia
pneumonia is more likely in elderly or chronic lung disease
most isolates produce beta lactamase
gram negative diplococci
causes otitis, sinusitis, bronchitis, bronchopneumonia
pneumonia is more likely in elderly or chronic lung disease
most isolates produce beta lactamase
question
legionella pneumophila
answer
what organism?
gram negative coccobacilli- gram stains poorly (silver stain is more effective)
intracellular- binds to CR3 complement and gets endocytosed but then phagolysosome fusion is inhibited and bacteria replicate in vacuole
ubiquitous in fresh water
catalase negative
cultured in BCYE agar (buffered charcoal yeast agar)
gram negative coccobacilli- gram stains poorly (silver stain is more effective)
intracellular- binds to CR3 complement and gets endocytosed but then phagolysosome fusion is inhibited and bacteria replicate in vacuole
ubiquitous in fresh water
catalase negative
cultured in BCYE agar (buffered charcoal yeast agar)
question
pontiac fever, legionnaire's disease
answer
legionella:
__________ acute onset of flu-like illness lasting several days and then resolving. possible hypersensitivity reaction
________ fever, GI illness, pneumonia, multiorgan failure. significant mortality
__________ acute onset of flu-like illness lasting several days and then resolving. possible hypersensitivity reaction
________ fever, GI illness, pneumonia, multiorgan failure. significant mortality
question
silver, BCYE, L-cysteine and iron salts
answer
legionella:
what stain is used to detect?
what type of agar is used to culture?
legionella requires _____ and ______ to grow
what stain is used to detect?
what type of agar is used to culture?
legionella requires _____ and ______ to grow
question
cell wall, PI, cold agglutinin, I antigen
answer
mycoplasma pneumoniae:
strict aerobe
no _______; not susceptible to Beta lactams
smallest free-living organism
adhesion protein ____ allows it to attach to respiratory epithelium and leads to destruction of cilia
major cause of respiratory infections
can do a ______ test to detect IgM antibodies that would bind the ________ on human RBCs at 4C
strict aerobe
no _______; not susceptible to Beta lactams
smallest free-living organism
adhesion protein ____ allows it to attach to respiratory epithelium and leads to destruction of cilia
major cause of respiratory infections
can do a ______ test to detect IgM antibodies that would bind the ________ on human RBCs at 4C
question
brucella
answer
what organism?
gram negative intracellular coccobacilli
zoonotic disease; can be from meat or dairy products
causes granulomas
gram negative intracellular coccobacilli
zoonotic disease; can be from meat or dairy products
causes granulomas
question
bartonella henselae
answer
what organism?
gram negative bacilli
inoculation by scratch or bite cat and then spreads to regional lymph node
can be seen in warthin-starry stain
gram negative bacilli
inoculation by scratch or bite cat and then spreads to regional lymph node
can be seen in warthin-starry stain
question
warthin starry
answer
what type of stain can be used for bartonella henselae?
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