question
complex growth media
answer
chemical composition is unknown
question
chemically defined (synthetic) growth media
answer
contain an exactly defined chemical composition; used for studies that focus on what molecules are required for growth; citrate agar/minimum salts
question
general-purpose growth medium
answer
used to grow a wide variety of non-fastidious bacteria; TSA (tryptic soy agar)
question
selective growth medium
answer
allow some types of bacteria to grow while inhibiting the growth of other types; blood agar
question
blood agar
answer
differentiates among bacteria based on their ability to break down red blood cells and hemoglobin
question
enrichment medium
answer
gives certain organisms a better chance at survival; blood or blood products, EMB (eosin methylene blue agar)
question
EMB (eosin methylene blue agar)
answer
inhibits the growth of Gram positive bacteria
question
all organisms will have the following:
answer
requirements for carbon, energy, macronutrients, micronutrients, vitamins/cofactors, water, and oxygen
question
good/vigorous growth
answer
organism grows well in medium
question
moderate growth
answer
organism grows in medium but no heavy growth
question
stunted growth
answer
organism doesn't grow well in medium and produces slight growth
question
no growth
answer
organism unable to grow in medium
question
two ways to separate a mixed culture
answer
dilution and isolation streak
question
streak plate
answer
getting a pure culture from a mixed culture by obtaining these well-isolated colonies
question
tryptic soy agar (TGA)
answer
general purpose complex growth medium used for streak plates
question
mannitol-salt agar (MSA)
answer
differential and selective growth medium
question
MSA is selective for
answer
staphylococci
question
eosin-methylene blue agar (EMB)
answer
differential and selective growth medium; contains peptone, lactose, sucrose, and dyes in eosin Y and methylene blue
question
EMB inhibits
answer
Gram negative bacteria
question
EMB differentiates based on
answer
ability to ferment lactose
question
what happens when organisms that cannot ferment lactose?
answer
produce colorless colonies
question
what happens when organisms ferment lactose?
answer
produce pink or purpose colonies
question
what happens when organisms ferment and produce large amounts of organic acids?
answer
produce colonies with green metallic sheen
question
growth interpretation (MSA)
answer
organism not inhibited by NaCl
question
growth identification (MSA)
answer
Staphylococcus, Micrococcus
question
no growth interpretation (MSA)
answer
organism inhibited by NaCl
question
no growth identification (MSA)
answer
not Staphylococcus
question
yellow halo interpretation (MSA)
answer
organism ferments mannitol
question
yellow halo identification (MSA)
answer
probably S. aureus
question
no yellow halo interpretation (MSA)
answer
organism does not ferment mannitol
question
no yellow halo identification (MSA)
answer
Staphylococcus species (other than S. Aureus); Micrococcus
question
no growth/stunted growth interpretation (EMB)
answer
organism inhibited by dyes
question
no growth/stunted growth identification (EMB)
answer
organism is Gram positive
question
good growth interpretation (EMB)
answer
organism not inhibited by dyes
question
good growth identification (EMB)
answer
organism is Gram negative
question
colorless growth interpretation (EMB)
answer
organism does not ferment sucrose or lactose
question
colorless growth identification (EMB)
answer
non-coliform
question
growth is pink and mucoid interpretation (EMB)
answer
organism ferments lactose and/or sucrose with some organic acid production
question
growth is pink and mucoid identification (EMB)
answer
coliform bacteria
question
growth is dark (purple to black with or without green metallic sheen) interpretation (EMB)
answer
organism ferments lactose and/or sucrose with large amounts of organic acid production
question
growth is dark (purple to black with or without green metallic sheen) identification (EMB)
answer
possible fecal coliform (E. coli)
Order an Essay Now & Get These Features For Free:
Turnitin Report
Formatting
Title Page
Citation
Outline
