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Gram stain is a differential stain because
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helps differentiate Gram positive from Gram negative bacteria
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Gram-positive bacteria
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stained purple/blue
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Gram-negative bacteria
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stained pink
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Gram negative bacteria
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...
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steps of staining
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-crystal violet, iodine, alcohol, safranin
-ALCOHOL is decolorized step important
-ALCOHOL is decolorized step important
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Acid Base Stain
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A differential stain to detect Mycobacteria, Nocardia, Cryptosporidium
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Mycobacteria
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-can be found in sputum specimens of patients affected by active or reactivated tuberculosis
-contains mycolic acid (not stain well with gram stain)
-contains mycolic acid (not stain well with gram stain)
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Nocardia
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lung disease nocardiosis
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Cryptosporidium
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diarrheal disease named cryptosporidiosis