question
what was the purpose of incubating the uninoculated plates?
answer
to observe jf bacteria grows. we can knkw if the plate was contaminated at any time in anyway by seeing if bacteria grows in incubation
question
three important factors typically included when describing bacterial growth
answer
incubation temperature, time, type of medium
question
failure to add iodine to gram stain
answer
cells will remain translucent
question
Failure to add decolorizer to gram staib
answer
both gram + and gram - remain colored
question
failure to add the safranin to gram stain
answer
the gram - cannot be distinguished from gram +
question
are acid fast negative cells stained, and by what?
answer
yes by carbolfuchsin
question
what benefit are capsules to cells?
answer
adhesion, protection of cell, prevents against phagocytosis
question
what is the purpose of emulsifying the sample in serum in capsule stain?
answer
for cells to stick to the slide instead of heat fixing
question
what does a positive result for endoscope stain indicate about the organism?
answer
the cell has the ability to produce spores, it is very resilient and tough
question
for a given antibiotic, is there a difference in susceptibility between gram + and gram - bacteria?
answer
gram - is less susceptible, more resistant, than gram +
question
zone of inhibition in a gram - bacteria, more resistant
answer
smaller zone of inhibition
question
how does antibiotic get from the disk into the agar?
answer
it diffuses from disk to agar
question
what is the general trend between bacterial death and UV exposure?
answer
longer exposure = less growth that is seen
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