Lab practicle

A glass beaker with a thin neck

Glass cylindrical container with an open mouth.

Long thin cylinder that is measured from the meniscus

Tool used to count groups of cell colonies

Tool used to sterilize lab equipment

Settings on an autoclave

Name, Date, Group, Media, Organism

MaConkey Agar

selective and differential media, SA: Bile Salts, DA: Lactose. Purple growth indicates lactose fermentation.

Mannitol Salt Agar

Isolates and ID's strains of staphylococcus, selective and differential, SA: NaCl(7.5%), DA: Mannitol, PH indicator: Phenol Red, Yellow growth indicates mannitol +, Pink growth indicates mannitol -

Snyders Test Agar

Used for the Snyders Test(Used in mouth bacteria experiment), selective and differential, detects presence of Lactobacilli, DA: Glucose, PH indicator: Bromcresol Green. Yellow growth indicates presence of Lactobacilli.

Thioglycolate Broth

Grows Anaerobic microbes, Biochemical, SA: Sodium Thioglycolate, O2 indicator: Methylene Blue, Growth at the bottom indicates an obligate Anaerobe.

Trypticase Soy Agar

Grows wide variety microbes, primarily done via a smear or streak inoculation(SOLID)

Trypticase Soy Broth

Grows a wide variety of microbes, General based media.(Liquid)

Test preformed to identify the pressence of Lactobacilli which are the cause of tooth decay.

Circular dot like growth pattern

Growth pattern in which the bacteria sprouts filaments

circular jaged edged growth pattern

Thin branch like growth pattern

Microbe that NEEDS oxygen to survive
EX: Psuedomonas Aeruginosa

Live throughout the medium, can tolerate the presence of oxygen.
EX: Staphylococcus Aureus

HATES oxygen, found at the bottom of the medium.
EX: Clostridium Butyricium

Surface membrane produced by organisms living at the top of the medium.

Clumps of growth in a medium

Cloudy growth throughout a medium

Escherchia Coli

Psuedomonas Aeruginosa

Clostridium Sporogenes

First step in focusing, reduces eye strain.

The second step in focusing, Helps with headaches.

Staining technique in which a dry heat fixed slide is stained with one dye and observed under a microscope.(Crystal violet, Methylene Blue, Safranin)

Staining technique where a heat fixed slide is stained with Nigorsin. Bacteria appear clear around a black background.

Staining technique which involves a primary stain of crystal violet, a mordant of Iodine, a decolorizer of acetone, and a counterstain of safranin.

A staining technique which uses a primary stain of Carbon Fuchin, a Decolorizer of acid alcohol, and a counter stain of methylene blue.

Mycolic Acid surrounded cells

Staining technique in which A primary stain of malachite green is used with a decolorizer of distilled water, and a counter stain of safranin.

Test done to determine if a bacteria is gram negative or gram positive. A drop of 3% KOH is mixed with a specimen.

Organism found in soil, produces bacitracin, laundry detergent enzymes and other important products. Resistant to heat, chemicals, and radiation.

Organism that is a very large Bacillus used because of its size.

Anaerobic organism both positive and negative, does not produce nuerotoxins but can cause intestinal water loss via the destruction of intestinal epithelial cells.

Gram negative rod that is a common resident of the digestive tract. its presence indicates fecal contamination. Causes urinary tract infections and food poisoning.

Aerobe gram negative rod. Oppurtunistic pathogen that is resistant to phagocytosis because of an outer slime layer. Causes eye and ear infections and is a known nosocomial infection.

Gram negative motile rod, lives in soil and water. commonly found in bathrooms and is pink and slimy in appearance. Often infects aids patients, burn victims, and is resistant to antibiotics.

Gram positive cocci with a grape cluster like structure. Salt tolerant and wide-spread, associated with skin and mucus membranes . Most common nosocomial infection. Causes food poisoning, toxic shock, scaled skin syndrome, pinkeye, and boils.

25C, 37C