question
Which of the following would be a positive result for the production of the enzyme coagulase?
answer
the formation of a clot in the test tube
question
True or False: The rapid Staphylococcus aureus test is based on antigen/antibody reactions.
answer
true
question
Which of the following organisms produces tryptophanase?
answer
E. Coli
question
Suppose you are given an unknown sample that you need to identify as either Salmonella or Shigella. To accomplish this, you decide to use the rapid ID test. When you added the Shigella antiserum to your sample, white clumps were produced while the Salmonella antiserum resulted in a cloudy sample with no clumps. Based on these results, what was the identity of your unknown?
answer
shigella
question
Which of the following organisms is resistant to the antibiotic novobiocin?
answer
staphylococcus saprophyticus
question
True or False: When trying to identify a bacterium as a member of Streptococcus Group A, B, C, or D, testing for susceptibility to the antibiotic bacitracin will give you sufficient information to identify the organism.
answer
false
question
Which of the following organisms causes β hemolysis on blood agar?
answer
staphylococcus aureus
question
Which of the following organisms ferments lactose?
answer
E. coli
question
What would a positive test for the rapid detection of Streptococcus pyogenes look like?
answer
two pink lines
question
Which of the following organisms utilizes citrate?
answer
salmonella
question
True or False: the only member in group B streptococcus is streptococcus agalactiae.
answer
true
question
staphylococci are ___________ while streptococci are ______.
answer
catalase positive; catalase negative
question
what is one disease that streptococcus pyogenes can cause?
answer
strep throat
question
in lab, we tested streptococcus pneumoniae and streptococcus viridans for susceptibility to:
answer
optochin
question
what is one disease that streptococcus pneumoniae can cause?
answer
pneumonia
question
Which group of streptococci is responsible for the formation of dental plaque?
answer
Viridans group
question
Which of the following organisms is a member of Group D streptococcus?
answer
Enterococcus faecalis
question
Which of the following test did you preform on your staphylococcus unknown (MSA Plate) last period?
answer
Coagulase
question
Which of the following tests did you preform on your Enterobacteriaceae unknown (MAC plate) last period?
answer
catalase and coagulase
question
True or False: to test for starch hydrolysis, the reagent iodine was added to the plate after incubation.
answer
true
question
Which of the following organisms is used as an indicator of fecal contamination of water?
answer
E. coli
question
The reagent used to test for phenylalanine deamination is _____; the reagent turns ___ as a positive result
answer
Ferric Chloride, green
question
What is the term that is used if water is coliform free and drinkable?
answer
potable
question
Which types of media did you use to isolate your unknown organisms?
answer
MacConkey agar and Mannitol salt agar
question
What is one illness caused by staphylococcus saprophyticus?
answer
urinary tract infection
question
which of the following organisms are never part of the normal flora of the skin, mucous membrane, and throat?
answer
staphylococcus saprophyticus
question
A negative test for the utilization of citrate would result in ________.
answer
a green color
question
True or False: shigella can cause typhoid fever.
answer
false
question
Which of the following is part of the definition for a coliform?
answer
fermentation lactose to acid and gas, gram negative, facultative anaerobes
question
A durham test is used to detect ____ produced by carbohydrate fermentation.
answer
gas
question
How does alpha hemolysis appear on blood agar?
answer
a green/brown zone surrounding the colony
question
The enzyme phenylalanase converts phenylalanine into
answer
ammonia and phenylpyruvic acid
question
True or False: a mannitol fermenter appears pink on mannitol salt agar (MSA)
answer
false
question
SIM media detects the production of hydrogen sulfide, indole production, and motility. A positive indole test indicates the enzyme _____ was produced by the bacterial strain
answer
tryptophanase
question
Carboyhydrate fermentation __________.
answer
May produce gas and produces acids
question
_______ is the enzyme responsible for digesting starch
answer
amylase
question
The urease converts urea into carbon dioxide and ______
answer
a. oxygen
b. starch
c. tryptophan
d. none of the above
d
b. starch
c. tryptophan
d. none of the above
d
question
A positive oxidase result _____
answer
bubbles
question
What color will a lactose fermenter appear on Eosin Methylene Blue agar (EMB)?
answer
green metallic
question
Partial hemolysis on blood agar plate is called _______
answer
alpha
question
Blood agar plate are ___ for hemolysis
answer
differential
question
Which type of media is not differential?
answer
phenylethanol agar
question
Phenylethanol agar is used ____________.
answer
Select for gram positive bacteria
question
True or False: MacConkey (MAC) agar is enriched with salt.
answer
false
question
Eosin Methylene Blue agar is selective for _________ and differential for _________.
answer
gram negative organisms, lactose fermentation
question
Mannitol Salt agar is selective for ______ and differential for _______.
answer
salt tolerant organisms, mannitol fermentation
question
which of the following types of agar would be the most helpful for isolating and differentiating staphylococcus aureus from other types of organisms
answer
mannitol salt agar
question
An __________ medium contains one or more compounds to distinguish between microbes in the medium. while an _________ medium contains one or more additives to encourage the growth of fastidious microbes.
answer
Differential, enriched
question
MacConkey agar is differential for ________ fermentation.
answer
lactose
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