Microbiology Lab Midterm

Where is the fire extinguisher?

Where is the security call button?

Where is the first aid kit?

Where is the eye wash station?

Where is the flashlight?

What are microbes that cause disease called?

What microbes do not cause disease (the vast majority of microbes)?

What microorganisms colonize the human body and are beneficial?

Polysaccharide derived from the cell walls of red algae.

To handle so as not to introduce any unwanted microbes.

A solidifying agent used in petri plates.

Environment containing oxygen.

The action of introducing microorganisms into environments where they can grow and reproduce.

What is the total magnification of the 4x scanning lens?

What is the total magnification of the 10x low power objective lens?

What is the total magnification of the 40x high power lens?

What is the total magnification of the 100x oil immersion objective lens?

What do we use to prevent refraction of light and observe fine details?

What is immersion oil?

What is the portion of the slide that is visible called?

What is cloudiness of the broth and is the best way to indicate growth?

When nutrients are seen floating on top of the nutrient broth, where are they growing?

What is the film formed on the top of the liquid broth called?

What is a mass of settled organisms at the bottom of the tube called?

What is the process of bacterial division called?

What do obligate arrows mean?

What is the color, smell, shape, margin, and elevation of a colony?

What are colonies considered to be if they do not touch each other?

What culture has more than one species of an organism present?

What culture demonstrates the presence of only one bacterial species where all bacterial cells comprising the culture are genetically identical?

Where do we transfer a colony for short term storage?

A new cell or microbiological culture made by transferring some or all cells from a previous culture to fresh growth medium.

What isotonic solution do we use in the lab?

What morphology can we see with the naked eye?

What morphology requires a microscope to see?

What cell arrangement does not exist?

What do we do to prevent overcrowding of bacteria or false positives?

What is the purpose of using liquid media to grow organisms?

Would you see isolated colonies on an inoculated slant?

Does bacteria cover the entire surface of a slant?

What characteristic must bacteria have to cover areas of an agar?

TNTC

TFTC

A sample that is diluted by a factor of 10 several, and often multiple, times to reduce the number of bacteria seen.

CFU/mL

A way of identifying organisms based on characteristics that can be determined by observation or experimentation.

What do we use to distinguish specific properties of cells?

Primary Stain, 60s

Mordant, 60s

Decolorizer, Less Than 20 Seconds

Counter Stain, 60s-120s

Gram +/-, Cell Morphology, Arrangement, Endospores (Only if Present)

What do endospores look like?

Only kills cells that are actively growing.

Kills active cells and dormant endospores at 121.5 degrees Celsius for 15 minutes.

Produces acid (turns yellow).

Remains purple.

Pressure chamber that generates hot steam for sterilization.

Used for treatment of small metal and glass items.

Used for treatment of liquids, nonmetallic objects, and biohazardous waste.

Disrupts DNA and RNA in living organisms, and is used by hospital and laboratory suppliers for materials that do not tolerate heat or chemical treatments. Also used in lunch meat.

Physically removes particulates from fluids. The size of the particles removed is determined by the pore size of the filter membrane.

Disinfectants applied to living tissue.

All blood, body fluids, secretions, excretions (except sweat), non-intact skin, and mucous membranes may contain transmissible infectious agents.

Intended to prevent transmission of infectious agents spread by direct or indirect contact with the patient or the patient's environment.

Intended to prevent transmission of pathogens spread through close respiratory or mucous membrane contact with respiratory secretions.

Prevent transmission of infectious agents that remain infectious over long distances when suspended in the air.

Test that determines whether or not a microorganism is susceptible to a relative amount of an antibiotic.

The area of media where bacteria are unable to grow.

How the body processes a drug.

The lowest concentration that prevents growth after overnight incubation.

The lowest concentration that completely kills all cells upon subculturing onto appropriate growth media.

Molecule that indicates infection, pregnancy, high cholesterol etc.

Two primary types of analytes.

Short-term treatment and protection.

Rapid slide agglutination procedure for detecting Staphylococcus aureus, which posses the virulence factors coagulase and/or protein A.

Binds to Fc portion of antibody.

Biochemical test used in diagnostics and research. A patient sample is washed with a specific antibody that is linked to an enzyme, which allows detection of any antibody that has bound to the immobilized antigen by the production of a color.

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