question
What is the stain used in simple staining
answer
Methylene Blue
question
What organisms were used in simple staining
answer
Staphylococcus aureus & Escherichia coli
question
What color are bacteria at the end of simple staining
answer
blue
question
What dye is used during negative staining
answer
Nigrosine
question
What organisms were used for negative staining
answer
Bacillus Megateriuim
question
what is the result of negative staining
answer
Dark background; colorless cell
question
Escherichia coli is Gram ____
answer
Negative
question
Moraxella Catarrhalis is Gram ____
answer
Negative
question
Bacillus Megaterium is Gram ____
answer
Positive
question
Micrococcus luteus is Gram ____
answer
Positive
question
Staphylococcus aureus is Gram ____
answer
positive
question
What 5 organisms were used in gram staining
answer
- Staphylococcus aureus
- Micrococcus luteus
- Bacillus Megaterium
- Moraxella Catarrhalis
- Escherichia coli
- Micrococcus luteus
- Bacillus Megaterium
- Moraxella Catarrhalis
- Escherichia coli
question
What bacteria was used for the Schaeffer-Fulton Spore Stain
answer
Bacillus megaterium
question
What is the primary stain in the Schaeffer-Fulton Spore Stain
answer
Malachite Green
question
What is the Mordant in the Schaeffer-Fulton Spore Stain
answer
Heat
question
What is the decolorizer in the Schaeffer-Fulton Spore Stain
answer
Distilled H2O
question
What is the counterstain in the Schaeffer-Fulton Spore Stain
answer
Safranin
question
What is the outcome for the Schaeffer-Fulton Spore Stain
answer
Pink bacteria/sporangium; Green spore
question
What bacteria was used for the Dorner Spore Stain
answer
Bacillus megaterium
question
What is the primary stain for the Dorner Spore Stain
answer
Carbolfuchsin
question
What is the mordant for the Dorner Spore Stain
answer
Heat
question
The Dorner Spore Stain does not have a decolorizer [True/False]
answer
True
question
What is the counterstain for the Dorner Spore Stain?
answer
Nigrosin
question
What is the outcome for the Dorner Spore Stain?
answer
Dark background; colorless sporangium; Fuchsia spore
question
What is the primary stain for the Ziehl-Neelsen Acid Fast Stain?
answer
Carbofuchsin
question
What is the mordant for the Ziehl-Neelsen Acid Fast Stain?
answer
heat
question
What is the decolorizer for the Ziehl-Neelsen Acid Fast Stain?
answer
Acid Alcohol
question
What is the counterstain for the Ziehl-Neelsen Acid Fast Stain?
answer
Methylene Blue
question
What bacteria are used in the Ziehl-Neelsen Acid Fast Stain?
answer
Mycobacterium smegmatis; Staphylococcus aureus
question
Mycobacterium smegmatis is Acid Fast ____
answer
Positive
question
Staphylococcus aureus is Acid Fast ____
answer
negative
question
What is the outcome color for a Acid Fast +
answer
Fuchsia
question
What is the outcome color for a Acid Fast -
answer
Blue
question
Spore forming diseases
answer
- Tetanus
- Botulism
- Gas Gangrene
- Anthrax
- C. Diff
- Botulism
- Gas Gangrene
- Anthrax
- C. Diff
question
What is the arrangement of S. aureus
answer
staphylococcus
question
what is the arrangement of E. coli
answer
single bacillus
question
what is the arrangement of bacillus megaterium
answer
Streptobacillus
question
what is the arrangement of micrococcus luteus
answer
tetrad cocci
question
what is the arrangement of Moraxella Catarrhalis
answer
diplococcus
question
what is the arrangement of mycobacterium smegmatis
answer
single bacilli
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