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economics

Microbiology Practical Stains

0 min read
Posted on 
May 22nd, 2023
Home economics Microbiology Practical Stains
question
Streak Plate
answer
The ____ method of isolation involves spreading a loopful of culture over the surface of the plate in 4 sections.
question
Spread plate
answer
The ____ method of isolation requires a previously diluted mixture of microorganisms be used.
question
Pour plate
answer
The ____ method of isolation requires a serial dilution with a loop or pipet.
question
Negative
answer
A ____ stain is used to determine the natural size and shape of bacteria, heat fixing is not used so the bacterial cell is not distorted.
question
Congo red
answer
The primary stain used in a negative stain is ____.
question
HCI
answer
No counterstain is used for a negative stain instead it is dipped in 1% ____.
question
Purple
answer
When using a gram stain the gram + bacteria will be ____.
question
Red
answer
When using a gram stain the gram - bacteria will be ____.
question
crystal violet
answer
The primary stain used for a gram stain is ____.
question
safranin
answer
The counter stain used for a gram stain is ____.
question
iodine
answer
A gram stain uses a mordant to lock in the primary stain to gram positive bacterial the mordant used is called Gram's ____.
question
ethyl alchohol
answer
A gram stain needs a decolorizer to remove the primary stain from gram negative bacteria, the decolorizer used is 95% ____.
question
acid fast
answer
An ____ stain is used the identify bacteria that are resistant to simple and gram staining.
question
Mycobacterium
answer
Acid-fast staining is required to stain bacteria with a thick waxy (lipoidal) wall, usually these bacteria are members of the ____ genus.
question
carbol fuchsin
answer
The primary stain used in an acid-fast stain is ____.
question
acid alcohol
answer
The decolorizer ____ is used in acid-fast stain to ensure the primary stain is removed from non-acid fast bacteria.
question
brilliant green
answer
The counterstains used for acid-fast stains are either ____ or methylene blue.
question
green
answer
In a spore stain the spores will remain ____ while the counterstain will stain the vegetative cells red.
question
bacillus
answer
____ bacteria usually produce spores when introduced to unfavorable conditions.
question
malachite green
answer
The primary stain used in Spore staining is ____.
question
safranin
answer
The counterstain used in Spore staining is ____.
question
acid fast
answer
Acid-Fast Stain, is B acid-fast or non acid fast?
question
gram positive
answer
Gram Stain, is this a Gram positive or a gram negative result?
question
yes
answer
Spore Stain, do the bacteria contain spores? (yes/no)
question
Capsule
answer
The ____ stain is used to identify bacteria with a capsule.
question
crystal violet
answer
The primary stain used for capsule staining is ____.
question
copper sulfate
answer
The decolorizer used for Capsule staining is 20% ____.
question
yes
answer
Capsule Stain, do the bacteria have capsules? (yes/no)
1 of 28
question
Streak Plate
answer
The ____ method of isolation involves spreading a loopful of culture over the surface of the plate in 4 sections.
question
Spread plate
answer
The ____ method of isolation requires a previously diluted mixture of microorganisms be used.
question
Pour plate
answer
The ____ method of isolation requires a serial dilution with a loop or pipet.
question
Negative
answer
A ____ stain is used to determine the natural size and shape of bacteria, heat fixing is not used so the bacterial cell is not distorted.
question
Congo red
answer
The primary stain used in a negative stain is ____.
question
HCI
answer
No counterstain is used for a negative stain instead it is dipped in 1% ____.
question
Purple
answer
When using a gram stain the gram + bacteria will be ____.
question
Red
answer
When using a gram stain the gram - bacteria will be ____.
question
crystal violet
answer
The primary stain used for a gram stain is ____.
question
safranin
answer
The counter stain used for a gram stain is ____.
question
iodine
answer
A gram stain uses a mordant to lock in the primary stain to gram positive bacterial the mordant used is called Gram's ____.
question
ethyl alchohol
answer
A gram stain needs a decolorizer to remove the primary stain from gram negative bacteria, the decolorizer used is 95% ____.
question
acid fast
answer
An ____ stain is used the identify bacteria that are resistant to simple and gram staining.
question
Mycobacterium
answer
Acid-fast staining is required to stain bacteria with a thick waxy (lipoidal) wall, usually these bacteria are members of the ____ genus.
question
carbol fuchsin
answer
The primary stain used in an acid-fast stain is ____.
question
acid alcohol
answer
The decolorizer ____ is used in acid-fast stain to ensure the primary stain is removed from non-acid fast bacteria.
question
brilliant green
answer
The counterstains used for acid-fast stains are either ____ or methylene blue.
question
green
answer
In a spore stain the spores will remain ____ while the counterstain will stain the vegetative cells red.
question
bacillus
answer
____ bacteria usually produce spores when introduced to unfavorable conditions.
question
malachite green
answer
The primary stain used in Spore staining is ____.
question
safranin
answer
The counterstain used in Spore staining is ____.
question
acid fast
answer
Acid-Fast Stain, is B acid-fast or non acid fast?
question
gram positive
answer
Gram Stain, is this a Gram positive or a gram negative result?
question
yes
answer
Spore Stain, do the bacteria contain spores? (yes/no)
question
Capsule
answer
The ____ stain is used to identify bacteria with a capsule.
question
crystal violet
answer
The primary stain used for capsule staining is ____.
question
copper sulfate
answer
The decolorizer used for Capsule staining is 20% ____.
question
yes
answer
Capsule Stain, do the bacteria have capsules? (yes/no)

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