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Streak Plate
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The ____ method of isolation involves spreading a loopful of culture over the surface of the plate in 4 sections.
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Spread plate
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The ____ method of isolation requires a previously diluted mixture of microorganisms be used.
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Pour plate
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The ____ method of isolation requires a serial dilution with a loop or pipet.
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Negative
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A ____ stain is used to determine the natural size and shape of bacteria, heat fixing is not used so the bacterial cell is not distorted.
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Congo red
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The primary stain used in a negative stain is ____.
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HCI
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No counterstain is used for a negative stain instead it is dipped in 1% ____.
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Purple
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When using a gram stain the gram + bacteria will be ____.
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Red
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When using a gram stain the gram - bacteria will be ____.
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crystal violet
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The primary stain used for a gram stain is ____.
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safranin
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The counter stain used for a gram stain is ____.
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iodine
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A gram stain uses a mordant to lock in the primary stain to gram positive bacterial the mordant used is called Gram's ____.
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ethyl alchohol
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A gram stain needs a decolorizer to remove the primary stain from gram negative bacteria, the decolorizer used is 95% ____.
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acid fast
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An ____ stain is used the identify bacteria that are resistant to simple and gram staining.
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Mycobacterium
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Acid-fast staining is required to stain bacteria with a thick waxy (lipoidal) wall, usually these bacteria are members of the ____ genus.
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carbol fuchsin
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The primary stain used in an acid-fast stain is ____.
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acid alcohol
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The decolorizer ____ is used in acid-fast stain to ensure the primary stain is removed from non-acid fast bacteria.
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brilliant green
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The counterstains used for acid-fast stains are either ____ or methylene blue.
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green
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In a spore stain the spores will remain ____ while the counterstain will stain the vegetative cells red.
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bacillus
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____ bacteria usually produce spores when introduced to unfavorable conditions.
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malachite green
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The primary stain used in Spore staining is ____.
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safranin
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The counterstain used in Spore staining is ____.
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acid fast
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Acid-Fast Stain, is B acid-fast or non acid fast?
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gram positive
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Gram Stain, is this a Gram positive or a gram negative result?
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yes
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Spore Stain, do the bacteria contain spores? (yes/no)
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Capsule
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The ____ stain is used to identify bacteria with a capsule.
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crystal violet
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The primary stain used for capsule staining is ____.
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copper sulfate
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The decolorizer used for Capsule staining is 20% ____.
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yes
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Capsule Stain, do the bacteria have capsules? (yes/no)