question
What could you observe on the ASN III + glucose plates?
answer
No growth due to catabolite repression (glucose repressing pathways)
question
What could we observe for the agar degraders on the SWC plate? FINISH
answer
...
question
What is the enzyme that degrades agar?
answer
Agarase
question
What is the gram morphology of purple non-sulfur bacteria?
answer
Gram negative coccobacilli to straight or helical rods
question
What is the unique feature about purple non-sulfur bacteria?
answer
They are capable of chemoheterotrophic growth when cultivated aerobically
question
What type of broth is the purple non-sulfur bacteria inoculated in?
answer
ATY broth
question
What is the propionic acid bacteria's major fermentation product?
answer
Propionic acid
question
Are propionic acid bacteria aerobes or anaerobes?
answer
Aeroduric (don't grow in the presence of oxygen), obligate anaerobes
question
What are the 2 genera of propionic acid bacteria?
answer
Cutibacterium and propionibacterium
question
What is the gram morphology of propionic acid bacteria?
answer
Gram positive diptheroids
question
Are propionic acid bacteria motile or non-motile?
answer
Non-motile
question
What is the colony morphology of Cutibacterium granulosa?
answer
Pink gumdrop colonies
question
Are propionic acid bacteria catalase positive or negative?
answer
Catalase positive
question
What is the colony morphology of Cutibacterium acnes?
answer
White, domed, gumdrop shaped colonies
question
What is the gram morphology of lactic acid bacteria?
answer
Gram positive rods or cocci
question
What is the major fermentation product of lactic acid bacteria?
answer
Lactic acid
question
Are lactic acid bacteria anaerobes or aerobes?
answer
Aerotolerant anaerobes (will still grow in the presence of oxygen)
question
What does nutritionally fastidious mean?
answer
The organism (lactic acid bacteria) have a variety of nutritional requirements that must be met by their plant or animal hosts
question
What do homolactic acid fermenters produce?
answer
Lactic acid as the only fermentation product, through the embden-meyerhorf glycolytic pathway
question
What do heterolactic acid fermenters produce?
answer
CO2, ethanol and lactic acid as the major fermentation products
question
What color does the bromcresol purple slant turn if lactic acid is produced?
answer
Purple to yellow -> if CO2 is also produced bubbles will form
question
What is the gram morphology of actinomycetes?
answer
Gram positive
question
Are actinomycetes aerobes or anaerobes?
answer
Obligate aerobes
question
What is the biggest actinomycetes genus?
answer
Streptomyces
question
What are hyphae?
answer
Individual actinomycete filaments
question
What are mycelium?
answer
Dense networks of hyphae
question
What are conidia?
answer
Spore like structures
question
What are sporophores?
answer
Hyphal structures bearing conidia
question
What is responsible for the earthy smell in dirt?
answer
Geosmins (volatile polycyclic carbohydrates)
question
What are the nitrogen and carbon sources for Czapek's agar?
answer
Sodium nitrate and sucrose
question
What is the gram morphology of the enterobacteria?
answer
Gram negative rods
question
Are enterobacteria anaerobes or aerobes?
answer
Facultative anaerobes
question
What are the types of enterobacteria that ferment lactose?
answer
Enterobacter, E. coli, Klebsiella
question
What is EMB agar selective and differential for?
answer
Gram negative rods & lac + versus lac -
question
What enterobacteria is indole positive?
answer
E. coli
question
Are enterobacteriaceae oxidase positive or negative?
answer
Negative
question
What is the gram morphology of the luminescent bacteria?
answer
Gram negative
question
What is bacteriophage lambda an example of?
answer
A temperate phage (partially lytic phage)
question
What is a lysongen?
answer
the host cell that contains prophage
question
What is a prohage?
answer
the integrated phage genome
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