question
what type of microscope do we use in lab
answer
parfocal
question
how do we determine final magnification
answer
ocular times objective
question
reagents used in a gram stain
answer
crystal violet
iodine
alcohol (ethanol)
safranin
iodine
alcohol (ethanol)
safranin
question
how is an organism "fixed" to the slide
answer
heat
question
gram positive organisms
answer
stain PURPLE
may be cocci or rods
may be cocci or rods
question
list gram positive organisms
answer
-staphylococcus pneumonia
-staphylococcus aureus
-streptococcus agalactiae
-bacillus species
-staphylococcus aureus
-streptococcus agalactiae
-bacillus species
question
gram negative organisms
answer
stain PINK
may be rods, cocci, diplococci, etc.
thick layer of phospholipids
may be rods, cocci, diplococci, etc.
thick layer of phospholipids
question
list of gram negative organisms
answer
escherichia coli
question
what is the most common agar that most specimens and isolates are plated to
answer
BAP
question
why is BAP commonly used in micro lab
answer
sheep blood helps bacterial growth and differentiation shows by hemolysis
question
BAP properties
answer
not selective
differential (due to 5% sheep blood)
differential (due to 5% sheep blood)
question
Mueller Hinton
answer
not selective
not differential
not differential
question
Macconkey agar
answer
selective for gram negative
(inhibits gram positive with bile salts)
differential (lactose fermenters vs. non)
(inhibits gram positive with bile salts)
differential (lactose fermenters vs. non)
question
mannitol salt agar
answer
selective for staphylococcus (7.5% NaCl)
differential for staphylococcus aureus (mannitol)
differential for staphylococcus aureus (mannitol)
question
Columbia Colistin Nalidixic Acid Agar (CNA)
answer
selective for gram positive
(nalidixic acid)
differential
(5% sheep blood)
(nalidixic acid)
differential
(5% sheep blood)
question
organisms that grow on MAC
answer
e. coli
proteus mirabilis
proteus mirabilis
question
organisms that grow on MSA
answer
staphylococcus aureus
staphylococcus epidermis
staphylococcus epidermis
question
organisms that grow on CNA plate
answer
staphylococcus aureus
question
organisms that grow on meuller hinton
answer
pseudomonas aeruginosa
e. coli
staphylococcus aureus
e. coli
staphylococcus aureus
question
organisms that grow on BAP
answer
everything
question
beta hemolysis
answer
complete clearing of agar
ex. staphylococcus aureus
ex. staphylococcus aureus
question
alpha hemolysis
answer
partial clearing of agar, greening of agar
ex. streptococcus pneumoniae
ex. streptococcus pneumoniae
question
non hemolytic
answer
no lysis/ clearing of agar
ex. proteus mirabilis
ex. proteus mirabilis
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