question
Neisseria
answer
Is a large genus of bacteria that colonize mucosa
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Only two pathogens neisseriae
answer
N. meningitidis
N. gonorrhoeae
N. gonorrhoeae
question
Neisseria
answer
Gram negative diplococci
Intracellular
Intracellular
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catalase and oxidase positive
Aerobic
Aerobic
answer
Neisseria
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Chocolate Agar non motile diplococci
answer
Neisseria
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N. gonorrhoeae
answer
causes gonorrhea
Sensitive dehydration
Piliated → attachment
Resistant phagocytosis
Sensitive dehydration
Piliated → attachment
Resistant phagocytosis
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gonorrhea
answer
belsoğukluğu
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antigenic variation
answer
N. gonorrhoeae
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Lipooligosaccharide (LOS) N.gonorrhoeae
answer
Typical gram negative outer membrane but not long o antigen LOS
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Outer membrane proteins (OMPs) Omp-opacity protein
answer
OMPII-ability to antigenic variation adhesion & attachment to the host cell
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N. gonorrhoeae virulence factors
answer
lgA protease on mucosal surface
Beta lactamase
Tissue invasion
Beta lactamase
Tissue invasion
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Thayer-Martin agar ( inhibit microbiots)
answer
N. gonorrhoeae
N. meningitidis
N. meningitidis
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Colistin
answer
Inhibits gram-
negative flora (N.
gonorrhoeae and N.
meningitidis resistant to
colistin, most saprophyic
species of Neisseria
susceptible)
negative flora (N.
gonorrhoeae and N.
meningitidis resistant to
colistin, most saprophyic
species of Neisseria
susceptible)
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Vancomycin
answer
Inhibits gram-positive flora
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Nystatin
answer
Inhibits yeast flora
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Trimethoprim
answer
Inhibits swarming Proteus
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Oxidase positive Catalase positive
answer
Neisseria
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N. gonorrhoeae treatment
answer
Cephalosporins → Ceftriaxone
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key test neisseria identification
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oxidase test neisseria
oxidase ( + )
oxidase ( + )
question
outer membrane proteins
N.gonorrhoeae
N.gonorrhoeae
answer
Por = nutrients enter the inside
Omp = antigenic variation, adhesion attachment
Rmp= associated with porin in the formation of pores aç kapa yapar antibiotikleri almaz
Omp = antigenic variation, adhesion attachment
Rmp= associated with porin in the formation of pores aç kapa yapar antibiotikleri almaz
question
Thayer-Martin agar
answer
Chocolate agar + Antibiotics.
neisseria için selective media.
neisseria için selective media.
question
Oxidase test
answer
have cytochrome oxidase
if purple blue = pozitive
if purple blue = pozitive
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whats the difference of N. meningitidis and N. gonorrhoeae?
answer
N. meningitidis capsulated.
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N. meningitidis virulence factors
answer
capsule
LPS endotoxin
LPS endotoxin
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meningitis
answer
fever. rigid neck. fatal if untreated. penicillin use.
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Septicemia (sepsis)
answer
LOS is responsible
Waterhouse-Friedrichsen syndrome
Disseminated intravascular coagulation = damar içi pıhtı
Waterhouse-Friedrichsen syndrome
Disseminated intravascular coagulation = damar içi pıhtı
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N. meningitidis lab diagnosis
answer
specimens blood and CSF
microbiota yok selective mediaya gerek yok
microbiota yok selective mediaya gerek yok
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both glucose and maltose
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N. meningitis
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only glucose. not maltoz sucroz. fruktoz
answer
N. gonorrhoeae
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N. meningitidis treatment
answer
- penicillin G is first choice
- chloramphenicol is second choice (for those allergic to penicillin)
- chloramphenicol is second choice (for those allergic to penicillin)
question
Morexella
answer
gram negative diplococci
ear infections /otitis media in infants and children, causing 15%-20% cases
ear infections /otitis media in infants and children, causing 15%-20% cases
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Moraxella özellikler
answer
Aerobic
oxidase ( + )
fastidious
oxidase ( + )
fastidious
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M. catarrhalis
answer
respiratory tract commensal normal microbiota 40% 50%
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Morexella and Neisseria
answer
N. gon glu +
N. men glu + mal +
M. cat DNase +
N. men glu + mal +
M. cat DNase +
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The most sensitive diagnostic test
answer
nucleic acid amplification test PCR
question
Which one of the following is not a virulence factor of
Neisseria gonorrhoeae?
Neisseria gonorrhoeae?
answer
Lipopolysaccaharide capsule
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