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halophilic organism
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extremophile that thrive in environments with very high concentrations of salt
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Sheep blood agar
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not a selective medium
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MacConkey agar (MAC)
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promote the growth of gram-negative organisms while inhibiting the growth of gram-positive organisms
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pressure, temperature, and time for autoclaving media
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15 lb above atmospheric pressure (psi), 121°C, 15 minutes
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Thayer-Martin agar
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promote the growth of Neisseria gonorrhoeae and Neisseria meningitidis
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three bacterial morphologies
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cocci, bacilli, spirilla
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cocci
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spherical or round
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bacilli
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straight and rod-shaped
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spirilla
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spiral-rod shaped
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Minimum inhibitory concentration (MIC)
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lowest concentration of antimicrobial agent that will visibly inhibit the growth of the organism being tested
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antiseptic
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chemical used to control the growth of microorganisms on living tissues
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disinfectant
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chemical used to kill or control the growth of microorganisms
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sterilization
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100% killing of microbes in or on a surface
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bactericide
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100% killing of microbes
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bacteriostasis
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reduces growth
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SIMs tests
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motility, H2S, and indole production
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camp test
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biochemical test used to differentiate Staphylococci from Streptococci
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biochemical test for oxidase production
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biochemical test used to differentiate Pseudomonas from Enterobacteriaceae
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coagulase test
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when rabbit plasma forms a fibrin clot, which is visible to the naked eye, when in the presence of the enzyme coagulase / Staphylococcus aureus will be positive
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Proteus spp.
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recognized by its spreading growth appearance on sheep blood agar