question
Non-selective vs selective for MSA and TSA
answer
TSA: nonselective, will gorw a wide variety of bacteria, yeasts and molds
MSA: selective for halotolerant organisms like staphylococci, differential ingredient is mannitol because organisms that ferment mannitol will produce acidic byproducts, turning the phenol red to yellow in color
MSA: selective for halotolerant organisms like staphylococci, differential ingredient is mannitol because organisms that ferment mannitol will produce acidic byproducts, turning the phenol red to yellow in color
question
How staph is spread
answer
Direct physical contact with bacteria, typically skin to skin
question
Staph aureus versus epidermis
answer
Aureus: catalase, Coagulase, and mannitol (fermentation) POSITIVE
Epidermis: catalase positive, Coagulase NEGATIVE, mannitol negative
Epidermis is a resident flora on skin
Epidermis: catalase positive, Coagulase NEGATIVE, mannitol negative
Epidermis is a resident flora on skin
question
Hemolytic reactions
answer
Alpha: damage (green halo) but not lysis of RBCs
Beta: lysis of RBCs (clear halo)
Beta: lysis of RBCs (clear halo)
question
Flora that is part of the URT and causes infections
answer
Rhinoviruses, adenoviruses, respiratory syncythial viruses
question
Throat sample procedure
answer
Use a tongue depressor whole collecting sample on a sterile saline swab
Transfer culture to one quarter of agar plate, use a loop in streak method
Incubate at 35 C for 24 hrs, then RT
Transfer culture to one quarter of agar plate, use a loop in streak method
Incubate at 35 C for 24 hrs, then RT
question
Causes strep throat
answer
Streptococcus pyogenes
question
Strep mutans
answer
Causes plague -
Hydrolyze sucrose into glucose and fructose. Glucose polymerized into dextran, which surrounds cells to form a glycocalyx. This attaches to the teeth, where it traps streptococci and lactobacilli.
Hydrolyze sucrose into glucose and fructose. Glucose polymerized into dextran, which surrounds cells to form a glycocalyx. This attaches to the teeth, where it traps streptococci and lactobacilli.
question
Acid production
answer
Streptococci and lactobacilli produce acids from glucose and fructose
Lactobacilli multiply at pH lower than 4.8, making enough acid to remove calcium from enamel. Snyder test measures acid production by lactobacilli, which cause dental carries.
Lactobacilli multiply at pH lower than 4.8, making enough acid to remove calcium from enamel. Snyder test measures acid production by lactobacilli, which cause dental carries.
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