question
What is Ubiquity
answer
being everywhere
question
What is a colony
answer
A cluster of cells or clone which arises from a single bacterium by asexual reproduction
question
What disease does Staphylococcus aureus cause
answer
toxic shock, food poisoning, abscesses
question
How is Staphylococcus aureus transmitted?
answer
Human to human contact, aerosols
question
What systems do Staphylococcus aureus affect?
answer
Digestive and integumentary
question
Staphylococcus aureus (Slide picture)
answer
Anthrax
question
What does Bacillus anthracis cause?
answer
Contact with wool or goat hair as well as contaminated meat
question
How does Bacillus anthracis transmit?
answer
Respiratory, Digestive, and integumentary
question
What systems do Bacillus anthracis effect
answer
pneumonia, urinary tract infection
question
Bacillus anthracis (Slide picture)
answer
aerosol droplets
question
What does Klebsiella pneumoniae cause?
answer
Respiratory, urinary
question
How does Klebsiella pneumoniae transmit?
answer
diphtheria
question
What systems does Klebsiella pneumoniae affect?
answer
Aerosol droplets
question
Klebsiella pneumoniae (slide picture)
answer
Respiratory, urinary
question
What does Corynebacterium diphtheriae cause?
answer
Syphilis
question
How does Corynebacterium diphtheriae transmit
answer
sexual contact
question
What systems does Corynebacterium diphtheriae effect
answer
reproductive
question
Corynebacterium diphtheriae (Slide picture)
answer
Allergies
question
What disease does treponema pallidum cause
answer
Aerosols
question
How does treponema pallidum transmit
answer
respiratory
question
What systems does treponema pallidum effect
answer
Aspergillosis, otomycosis
question
treponema pallidum (Slide picture)
answer
Spore aerosol
question
What disease does Rhizopus nigricans cause
answer
Respiratory, nervous
question
How does Rhizopus nigricans transmit
answer
Mycobacterium because of mycolic acid
question
What system do Rhizopus nigricans effect
answer
Acid-fast bacteria contain mycolic acid, a waxy material found in their cell wall
question
Rhizopus nigricans (Slide picture)
answer
a simple stain that stains the background but leaves the bacteria unstained
question
What disease does Aspergillus niger cause
answer
A stain that gives color to the cell or target.
question
How does Aspergillus niger transmit
answer
It is a test to determine if a bacteria is gram positive or gram negative.
question
What system does Aspergillus niger effect
answer
purple
question
Aspergillus niger (Slide picture)
answer
pink
question
What bacteria stain acid-fast
answer
red
question
Why do some bacteria stain acid-fast
answer
blue
question
What is a negative stain
answer
A stain that is used to indicate Spore formation
question
What is a positive stain
answer
green
question
What is a gram stain
answer
organisms that breakdown glucose in the absense of oxygen.
question
What color is gram positive?
answer
cannot survive with oxygen
question
What color is gram negative?
answer
can live with or without oxygen
question
What color does acid fast stain
answer
Fluid thioglycollate
question
What color does non acid fast stain
answer
•Fluid thioglycollate is used to determine the oxygen requirements of bacteria.'
Bacteria on the top need oxygen, on the bottom don't, throughout can or can not
Bacteria on the top need oxygen, on the bottom don't, throughout can or can not
question
What is the Schaeffer-Fulton Stain used for?
answer
if it affects the bacteria to too large an extent it may rupture or shrink
question
What color indicates a endospore
answer
Stores genetic material in harsh conditions and facilitates survival. Once conditions are restored it goes back to being a vegetative cell. ONLY PROKARYOTIC
question
What are anaerobes?
answer
fungal reproductive bodies
question
What are obligate anaerobes?
answer
Made from an enzymatic digest of protein and soy
Supplies vitamins and growth factors to organisms
Supplies vitamins and growth factors to organisms
question
What are facultative anaerobes?
answer
Selective medium that permits growth of gram negative bacteria
is also differential as it contains sugar lactose: organisms that ferment lactose are purple, cant produce pink
is also differential as it contains sugar lactose: organisms that ferment lactose are purple, cant produce pink
question
What medium is used to grown bacteria based on oxygen needs
answer
A synthetic medium of inorganic salts and usually glucose
only organism that can make all their cellular components from glucose and inorganic salts can grow
only organism that can make all their cellular components from glucose and inorganic salts can grow
question
What is fluid thioglycollate
answer
In a standard plate count, the number of colony forming units (cfu) is determined.
question
How does osmotic pressure affect bacterial growth
answer
sample/ (diluent + sample)
question
What are endospores
answer
dilution x dilution
question
what are fungal spores
answer
number of colonies on plate x 1/vol of sample x 1/dilutionWhat
question
What is tryptic soy agar?
answer
cloudiness of water
question
What is Eosin Methylene Blue Agar?
answer
the amount of light that passes through the sample and reaches the detector
question
What is glucose salts agar
answer
A measure of how quickly light can travel through a material - the higher the optical density, the slower light waves travel through it
question
What is the standard plate count
answer
unicelluar or multicellular
question
What is the formula for single dilutions
answer
Prefect
question
What is the formula for serial dilutions
answer
Imperfect
question
What is the formula for number of colony forming units(cfu) in original sample
answer
2-(log%transmittance)
question
What is turbidity
answer
Colony forming units
question
What is percent transmittance
answer
Special stains (Capsule/Flagella) and Negative
question
What is optical density
answer
India ink
question
What is cell number
answer
methylene blue
question
Is Rhizopus nigricans perfect or imperfect
answer
Crystal violet, Gram's iodine, safranin
question
Is Aspergillus niger perfect or imperfect
answer
The primary stain
question
What is the formula for optical density
answer
Fixative
question
What is CFUs
answer
Decolorizer
question
What stains do not require heat fixing
answer
secondary stain
question
What stain is used in negative stain
answer
Malachite green, safranin
question
What stain is used in positive stain
answer
E.coli
question
What stains are used in gram stains
answer
undefined
question
What is the purpose of crystal violet gram staining
answer
undefined
question
What is the purpose of Iodine in staining
answer
undefined
question
What is the purpose of ethyl alcohol in staining
answer
undefined
question
What is the purpose of safranin in staining
answer
undefined
question
What dyes are used in endospore stain
answer
undefined
question
What grows green on Eosin methylene blue agar
answer
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