question
Sheep Blood Agar (SBA)
answer
- Supports everything besides very fastidious bacteria
- Has different types of hemolysis (beta, alpha, and gamma)
- Nutritive and differential
- Has different types of hemolysis (beta, alpha, and gamma)
- Nutritive and differential
question
Chocolate Agar (CAP)
answer
- Same as SBA but the cells have been lysed which releases X factor and V factor that support fastidious organisms
- Preferred by N. gonorrhea and Haemophilus spp.
- Preferred by N. gonorrhea and Haemophilus spp.
question
MacConckey Agar
answer
- Selective and differential
- Allows for the growth of gram negative RODS only
- If they ferment lactose the colonies are PINK
- Inhibits swarming of Proteus spp.
- Allows for the growth of gram negative RODS only
- If they ferment lactose the colonies are PINK
- Inhibits swarming of Proteus spp.
question
Hektoen Enteric Agar (HE)
answer
- Selective and differential
- Bile salts and dye slow the growth of nonpathogenic gram negative bacteria allowing Salmonella and Shigella to grow
- If it ferments lactose (nonpathogenic) it produces SALMON colonies
- H2S produced by Salmonella turns agar black
- Bile salts and dye slow the growth of nonpathogenic gram negative bacteria allowing Salmonella and Shigella to grow
- If it ferments lactose (nonpathogenic) it produces SALMON colonies
- H2S produced by Salmonella turns agar black
question
Phenylethyl Alcohol Agar (PEA)
answer
- Inhibits gram NEGATIVE bacteria
- Smells like flowers, flowers are positive, gram positive bacteria grow on this agar
- Smells like flowers, flowers are positive, gram positive bacteria grow on this agar
question
Thayer-Martin Modified Agar (MTM)
answer
- Enriched and selective for N. gonorrhoeae and N. meningitidis
- Colistin: antibiotic that inhibits gram negative bacteria
- Vancomycin: inhibits gram positive bacteria
- Nystatin: inhibits yeast
- Colistin: antibiotic that inhibits gram negative bacteria
- Vancomycin: inhibits gram positive bacteria
- Nystatin: inhibits yeast
question
Columbia CNA with Blood
answer
- Three peptone sources and 5% sheeps blood to make hemolysis visible
- CNA = colistin and nalidixic that stop growth of gram negative organisms
- CNA = colistin and nalidixic that stop growth of gram negative organisms
question
Gram Negative Broth
answer
- Used to cultivate gram negative pathogens such as salmonella and shigella from stool and recital samples
- Sodium citrate and sodium desoxycholate inhibit gram positive and nonenteric gram negative
- Subculture after 6-8 hours
- Sodium citrate and sodium desoxycholate inhibit gram positive and nonenteric gram negative
- Subculture after 6-8 hours
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Thioglycolate Broth
answer
- Most common and useful
- .075% agar can prevent oxygen throughout broth --> anerobic environment with thyioglycolic acid
- .075% agar can prevent oxygen throughout broth --> anerobic environment with thyioglycolic acid
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Nutritive Agar
answer
Contains nutrient that support growth without giving an organism an advantage
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Selective Agar
answer
Contains agents (dyes, bile salts, alcohols, acids or antibiotics) that are inhibitory to all organisms except those being sought
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Differential Agar
answer
Employs some factor that allows colonies of a species to exhibit certain metabolic or culture characteristics that can be used to distinguish them from something on the same plate (lactose fermentation, hemolysis)
question
Enrichment Agar
answer
Provides specific nutrients required for the growth of particular bacterial pathogens
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