question
WLN
answer
- yeast extract, tryptone, glucose
- plate yeast
- can also use anti-bacterial compounds, antibiotics such as chloramphenicol, to the media to discourage bacterial growth
- plate yeast
- can also use anti-bacterial compounds, antibiotics such as chloramphenicol, to the media to discourage bacterial growth
question
MRS
answer
- beef extract
- plate bacteria
- can also add an anti-fungal agent, such as Delvocid, to discourage yeast/mold growth
- plate bacteria
- can also add an anti-fungal agent, such as Delvocid, to discourage yeast/mold growth
question
WLD
answer
- WLN with Actidione (a.k.a cycloheximide)
~ will prevent the growth of Sacch
- plate yeast
~ will prevent the growth of Sacch
- plate yeast
question
MLAB
answer
- MRS, fructose, malic acid
question
Separation of yeast and bacteria on plates
answer
- can be facilitated by use of antibiotics
- use of acid- plates
- use of Actidione.
- use of acid- plates
- use of Actidione.
question
cell count equation
answer
(average cell count x 25)/ 10^-4
question
% viability equation
answer
viable cells/ viable + dead cells
question
Kohler illumination
answer
- setting the microscope up
question
Yeast strains
answer
- Brettanomyces bruxellensis
- Hanseniaspora uvarum
- Pichia membranifaciens
- Saccharomyces cerevisiae
- Schizosaccharomyces pombe
- Zygosaccharomyces bailii
- Metschnikowia pulcherrima
- Hanseniaspora uvarum
- Pichia membranifaciens
- Saccharomyces cerevisiae
- Schizosaccharomyces pombe
- Zygosaccharomyces bailii
- Metschnikowia pulcherrima
question
Bacteria strains
answer
- Gluconobacter oxydans
- Bacillus megaterium
- Lactobacillus casei
- Lactobacillus plantarum
- Oenococcus oeni
- Pediococcus parvulus
- Bacillus megaterium
- Lactobacillus casei
- Lactobacillus plantarum
- Oenococcus oeni
- Pediococcus parvulus
question
1/2 MRS
answer
- MRS diluted 1:1 with deionized water
question
Tip plate calculation
answer
(number of cells in lane) / (volume pipetted onto lane in mL) / (lane dilution (ex: 10^-4)) = cfu / mL
Order an Essay Now & Get These Features For Free:
Turnitin Report
Formatting
Title Page
Citation
Outline
