Module 7

Before you place the filter disks on the plates, how will you inoculate the plates with bacteria in Exercises 6-7 and 6-8?

Penicillin is an antibiotic made by

Subculturing

The total elimination of the normal microbiota of the mouth

In exercise 6-9,when you transform bacterial cells with the plasmid expressing the gene coding for ampicillin resistance, you will see growth on ampicillin-containing agar plates after incubation. Furthermore, the cells will flouresce.

A sample of fresh ground beef was inoculated onto EMB agar. Following incubation, colonies with a light yellowish color were seen. This result is an indication there was fecal contamination of this beef.

Bacteria and fungi that commonly produce antibiotics usually produce the antibiotics during sporulation.

In Exercise 7-4 you will use a simulated blood-type assay to examine

The relationship between humans and Eschericia coli is usually considered as mutualistic.

Why is HCl poured on the DNase test agar plates that you inoculated last period?

Bacteria were inoculated onto blood agar plates to test for the production of hemolysins. Which of the following results would indicate that the bacteria produces hemolysins?

You will need to incubate your test wells for 15 minutes twice. Which two of the following describe the conditions under which you will incubate the wells?

ELISA stands for

In our simulation of an epidemic, what is the "agent" of disease?

In the precipitin test BSA and anti-BSA antibodies were added to a capillary tube. How can you tell that an antibody-antigen interaction had taken place?

E. coli 363 is susceptible to T4 bacteriophage since ______ appeared on the spread plates of E. coli 363 and T4 bacteriophage.

An organism is inoculated onto a DNase plate. Following incubation, HCl is added and a clearing is seen around the growth of the organism. This indicates that the organism

Which one of the following describes the appearance of alpha-hemolysis on blood agar?

The relationship between humans and the bacteria that are part of their normal microbiota

A prepared slide of a macrophage is observed under the microscope. The macrophage contains many small, dark purple stained spheres. These are likely

symbiosis

commensalistic

mutalitsitc

parasitic

Benefits are often

e. coli helps with production and uptake of

opportunistic infections

parasites can also be described as

legumes and rhizobiums

bacteria parasites

five major stages of lytic phage eplication

attachment of phage is

plaques

calculate concentration of phage

DNase

Fibrinogen

coagulase

hemolysins

alpha hemolysins

beta hemolysins

gamma hemolysins

HCl

Blood agar hemolysin results

antibodies made up of

Precipitin reaction

Agglutination reaction

complement fixation

Our preciptin test uses