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AxiChrom™ 300-1600 columns
Operating Instructions
Original instructions
2.3 Emergency procedures
Introduction
This section describes how to shut down the AxiChrom Master in an emergency. The
section also describes the result in the event of power failure.
Emergency shutdown of
AxiChrom Master
In an emergency situation, press the emergency stop button on the AxiChrom Master
to stop adapter movement.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 3
5
2 Safety instructions
2.3 Emergency procedures
2.5 Crush injury risks
Introduction
This section describes areas within safety zone A where particular care must be taken
to avoid the risk of crush injury to fingers or hands when different parts of AxiChrom
columns are in motion.
Specific areas to avoid
Keep fingers and other body parts away from the following areas when column parts
are in motion.
Crush injury risk areaMovement
Adapter moving up
40 AxiChrom 300-1600 columns Operating Instructions 29065430 A
D
2 Safety instructions
2.5 Crush injury risks
Crush injury risk areaMovement
Adapter moving up
Adapter moving down
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 4
1
2 Safety instructions
2.5 Crush injury risks
Crush injury risk areaMovement
Adapter moving down
Column tube moving
down
42 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
2 Safety instructions
2.5 Crush injury risks
Crush injury risk areaMovement
Column tube swinging in-
wards
• Crush risk between
column tube and bot-
tom distribution as-
sembly (shown in im-
age)
• Crush risk between
column tube and
adapter (not shown)
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 4
3
2 Safety instructions
2.5 Crush injury risks
Intelligent Packing
Chromatography media is packed in the column using axial compression achieved by
the column servo motor. Packing can be controlled using the appropriate AxiChrom
Master wizard functions. Packing can also be performed and controlled using the
UNICORN Intelligent Packing function of an ÄKTAprocess system for column sizes 300
to 1000.
ÄKTAprocess UNICORN Intelligent Packing functions are currently not com-
patible for use with AxiChrom 1200-1600 columns as standard. Contact your
local GE representative for more information about this.
Note:
Main components
1
4
5
6
3
9
8
10
11
141312
7
2
50 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
3 System description
3.1 Column description
AxiChrom300-1600Mastermain
components
1
2
3
4
5
6
7
8
FunctionPart
EMERGENCY STOP button1
User interface touch screen2
Rinse nozzle pneumatic control switch (for supply of air to the Rinse nozzle)3
Lockable wheels4
Electrical cabinet (at the rear of the AxiChrom 300-1600 Master)5
Pneumatic connections for supply of air to the media valve (black) and
rinse nozzle (blue)1
6
Connection panel (at the rear of the AxiChrom 300-1600 Master)7
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 53
3 System description
3.2 AxiChrom 300-1600 Master description
For information on column weights and dimensions, seeColumndimensions, on page3
14
and Column weights, on page 315.
The illustration below shows how the column should be moved. Carefully read and follow
the labels and symbols on the column. For information on wheel kits, see Section 4.4
Wheel kits, on page 63.
A B
C
D E
F
FunctionPart
The center of gravity should be centered over the forks (red in illustration)
of the lifting equipment.
A +
B
Shows where not to lift.C
The labels indicate how to place the forks. D: Centralized on sizes 450 to
1000. F: Shifted to the left on sizes 300 and 400.
D + F
The label indicates the center of gravity.
E
Transport in crate: 1200-1600
columns
For information on how to transport the crates, refer to theAxiChrom1200-1600 columns
Unpacking Instructions.
60 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
4 Installation
4.2 Transport
Moving 1200-1600 columns
After unpacking from the transport crate, AxiChrom 1200-1600 columns can be moved
to the intended process position by lifting using eyebolts and an overhead crane or hoist.
Alternatively AxiChrom 1200-1600 columns can be rolled over an even surface using
a
wheel kit that is available as an optional accessory for the column, see Wheel kits for
AxiChrom 1200-1600 columns, on page 63, or the columns can be moved using other
suitable capacity moving equipment, such as air castors.
NOTICE
Do not place any type of lifting or moving equipment under the
column feet.
Please note that the columns are heavy and cannot be easilymoved. Carefully
read the Site Preparation Guide and Unpacking Instructions before placing
the column.
Note:
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 61
4 Installation
4.2 Transport
Connections on the column
The illustration below shows the connection points on the column.
12
13
17161514
18
19
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 69
4 Installation
4.6 Connections
6.2 General maintenance tools
This section describes general tools required for the maintenance of all sizes of AxiChrom
columns.
Tool kit
General tools required for disassembly, maintenance and assembly of AxiChrom columns
are provided in an AxiChrom toolkit, available from GE. For further details, see Section 12.7
Ordering information, on page 328.
O-ring removal tool
The removal tool is used for removing O-rings from their grooves and for similar tasks.
The tool is made of plastic to avoid scratching O-ring fittings. The image below shows
how to use the removal tool to remove the snap ring used with plastic bed supports.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 111
6 General maintenance and service
6.2 General maintenance tools
Plastic mallet
The plastic mallet is used to tap the snap ring gently into position when fitting plastic
bed supports. The groove on the mallet head may be used to lever the snap ring over
the edge of the bed support if the fit is tight. The image below shows how to use the
mallet head to lever the snap ring into position.
PTFE tape
It is recommended to wrap PTFE tape around the threads of all bolts and screws of size
M8 and above, with the exception of bolts that go into a threaded insert.
In maintenance procedures that involve the removal of bolts and screws with PTFE tape,
remove the old tape from the bolts and apply new tape before refitting the bolts and
attaching the nuts. Apply the tape in the same direction as the threads so that refitting
the nuts draws the tape into the threads.
112 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.2 General maintenance tools
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 1
17
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
Column disassembly wizard
TheMaintenancemenu wizard dialog screens that the user sees are slightly
different for AxiChrom300-1000 columns andAxiChrom1200-1600 columns.
This is due to the extramaintenancewizard steps that allow the user to perform
media valve maintenance on AxiChrom 1200-1600 columns.
Note:
118 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Precheck: Make sure that the inlet and outlet flow paths are open and that
the column is fully depressurized (for example by disconnecting the Top
mobile phase hose).
Press Confirm.
2
CAUTION
A sudden release of pressure when disconnecting
tubing may cause injury.
Precheck: Verify that the four locking pins and all lock blocks are in their
storage positions as illustrated below. The illustration shows four blocks. The
number of blocks differs between column sizes.
3
Press Confirm.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 1
21
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Unscrew and remove the nuts from the top column flange bolts as illustrated
below (note that the number of bolts differs between column sizes).
4
NOTICE
Be careful not to damage the column tube when re-
moving the nuts. Use the closed end of the wrench to
remove the nuts.
Press Confirm.
Verify that all top flange nuts are removed. Press Confirm to move the
adapter to the top position.
5
Result: The adapter moves to its top position.
NOTICE
If all nuts are not removed, the column may be dam-
aged in later steps.
122 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Remove the lock blocks from their storage positions. Fit the lock blocks in
the lock positions as illustrated below (the illustration shows four blocks in
lock positions 1A, 1B, 1C and 1D: the number of blocks differs between col-
umn sizes). Tighten the bolts securely to 45 Nm, using a torque wrench. Press
Confirm.
6
NOTICE
Do not use force to fit lock blocks. Lock blocks that are
difficult to screw into place may be damaged and must
be replaced.
Verify that the lock blocks are securely fitted. Press Confirm to start lifting
the top unit assembly.
7
Result: The top unit now moves upward.
NOTICE
Lifting the top unit with incorrectly placed lock blocks
may damage the column.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 1
23
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Insert and secure the locking pins in the lock hole positions 2A and 2B, as
illustrated below.
8
NOTICE
Moving the adapter with only one locking pin in place
may damage the column.
If the locking pins cannot be inserted into the lock holes for 300-600 columns,
the top unit may not be properly seated on the lock blocks as shown below.
Shake the top unit gently by hand until the adapter rests securely on the
lock blocks, as shown below.
124 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Verify that the locking pins are correctly inserted and secured in position.
Press Confirm to move the adapter up to the chamfer, in the top of the col-
umn tube, to release the compression of the adapter seals.
9
Result: The adapter moves up to the column tube chamfer.
Unscrew the nuts and remove the bottom flange bolts, using two wrenches
(one on either side of the bolt and nut assembly). Press Confirm.
10
Verify that all bottom flange bolts are removed. Press Confirm to lift the
column tube.
11
Result: The column tube now moves up.
NOTICE
If all bottom flange bolts are not removed, the column
may be damaged in later steps.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 125
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Insert and secure a locking pin in the lock hole position 3, as illustrated below.
Press Confirm.
12
NOTICE
Make sure to insert the safety peg into the locking pin
so that the safety catch is at the bottom of the locking
pin, as shown in the illustrations. Insertion in any other
orientation may result in damage to the safety peg
and locking pin.
1200-1600 columns300-1000 columns
Verify that the locking pin is secured and in the correct position. Press Con-
firm to lower the adapter.
13
Result: The adapter moves down to release the weight from the lock blocks.
126 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Remove the lock blocks from their lock positions and place the blocks in
their storage positions. Press Confirm.
14
Verify that all lock blocks are removed and in their storage positions. Press
Confirm to move the adapter to the top position.
15
Result: The adapter now moves to its top position.
Swing out the column tube to perform the maintenance operations. Use the
remaining locking pin to secure the column tube in the maintenance position.
16
CAUTION
Make sure that no body parts become trapped be-
tween the column tube assembly and the column
frame when using the swing out function for mainte-
nance work on the column. The column tube must be
locked in place during all maintenance work. Due to
the weight of the column tube assembly, it is not advis-
able to try to stop the momentum of the assembly by
hand when it is set in motion.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 127
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
If you are going to perform maintenance operations related to the media
valve on a 1200, 1400 or 1600 sized column, you have to lift the bottom
backing plate to be able to access the media valve. See Lifting the bottom
backing plate, on page 215.
17
Column assembly wizard
ActionStep
If you have performed maintenance operations related to the media valve
on a 1200, 1400 or 1600 sized column, you have to lower the bottom backing
plate before starting the assembly. See Lowering the bottom backing plate,
on page 223.
1
128 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Press Confirm.3
Result: The adapter will move to the top position if it is not already in the top
position.
Note:
The adapter will already be in the top position if the adapter has not been
moved after column disassembly.
Precheck: Make sure that the inlet and outlet flow paths are open. Press
Confirm.
4
Verify that the lock blocks are in their storage positions as illustrated below.
The illustration shows four blocks. The number of blocks differs between
column sizes. Press Confirm.
5
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 131
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Remove the locking pin 4, that secures the column tube in maintenance
position. Press Confirm.
6
Swing the tube in position for operation. Press Confirm.7
132 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Insert bottom flange bolts, with their corresponding washers, to align the
tube with the corresponding holes in the column bottom lid, as illustrated
below. Do not refit the nuts yet. Press Confirm.
8
Verify that all bottom flange bolts are inserted and that the column tube is
properly aligned. Press Confirm to move the adapter.
9
Result: The adapter moves down into position so that the lock blocks can
be fitted.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 133
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Remove the lock blocks from their storage positions. Fit the lock blocks in
the lock positions as illustrated below (the illustration shows four blocks in
lock positions. The number of blocks differs between column sizes. Tighten
the bolts firmly. Wet the scraper seals with 20% ethanol. Press Confirm.
10
NOTICE
Lifting the column tube with incorrectly fitted lock
blocks may damage the column.
NOTICE
Do not use force to fit lock blocks. Lock blocks that are
difficult to screw into place may be damaged and must
be replaced.
Verify that the lock blocks are securely fitted. Press Confirm to start lifting
the column tube.
11
Result: The column tube moves upward.
134 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Remove the locking pin from lock hole position.12
Place the locking pin in storage position as illustrated below.
Press Confirm.
NOTICE
Moving the column tube with a locking pin in the wrong
place may damage the lock blocks.
Verify that the locking pin is removed and placed in storage position. Press
Confirm to lower the column tube.
13
Result: The column tube moves downward.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 135
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Fit the flange nuts and washers to the bottom flange bolts, and finger
tighten the nuts. Press Confirm.
14
Verify that all bottom flange bolts are properly fitted. Press Confirm to lift
the top unit assembly.
15
Result: The top unit moves upward.
136 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Remove the locking pins from lock hole positions 2A and 2B.16
Place the locking pins in their storage positions as illustrated below.
Press Confirm.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 137
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Tip:
If the locking pins are difficult to remove, the top unit may not be properly
seated on the lock blocks. If this happens for smaller columns with compara-
tively lightweight top units (300-600 column sizes only), shake the top unit
gently by hand until the adapter rests securely on the lock blocks, as shown
in the image.
Verify that the locking pins are removed from both positions and placed in
their storage positions. Press Confirm to lower the top unit.
17
Result: The top unit moves downward to position.
Remove the lock blocks from their lock positions and place the blocks in
their storage positions under the top flange as illustrated below The illustra-
tion shows four blocks. The number of blocks differs between column sizes.
Press Confirm.
18
NOTICE
Moving the adapter with a lock block in the wrong
place may damage the column.
138 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Verify that the threads of the top flange bolts are wrapped with PTFE tape
and that all lock blocks are removed and in their storage positions. Press
Confirm.
19
Fit the washers and nuts to the top flange bolts as illustrated below. Finger
tighten the nuts. Press Confirm.
20
Verify that all top flange bolts are properly fastened. Press Confirm to lower
the adapter.
21
Result: The adapter is pressing the column tube downward.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 139
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Using a calibrated torque wrench, tighten the bottom flange nuts, crosswise
and in increments, to the correct tightening torque, according to the following
torque table. Press Confirm.
22
Tightening torque [Nm]
for flange nuts
Stainless steel tubes
Tightening torque [Nm]
for flange nuts
Acrylic tubes
Column size
8080300
8080400
8080450
100100600
140140800
1401401000
1801801200
3003001400
3003001600
Verify that all bottom flange bolts are properly tightened. Press Confirm to
lower the top unit.
23
Result: The adapter pulls the top unit downward.
Using a calibrated torque wrench, tighten the top flange nuts to the correct
tightening torque (refer to the torque table in step 22, above). PressConfirm.
24
Verify that all top flange bolts are properly tightened. Press Confirm.25
140 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.4 Putting the column in and out of maintenance mode
ActionStep
Set the media valve to Open in the Manual Control wizard in the AxiChrom
300-1600 Master.
1
F F
E
E
E
E
AB C D
FunctionPart
Valve pneumatic inlets
A
Slurry inletB
Bottom mobile phase inlet/outletC
Rinse inletD
Media valve bolts screwed into the bottom backing plateE
Locking screws on the tube holder boltsF
Disconnect the air supply tubes from the two valve pneumatic inlets (A) on
the media valve.
Note:
Make sure tomark each of the supply tubes before disconnection, for example
with a piece of tape, so that these can be reconnected correctly.
Disconnect the hoses to the:
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 143
6 General maintenance and service
6.5 Media valve maintenance
ActionStep
• Slurry inlet (B),
• Bottom mobile phase inlet/outlet (C), and
• Rinse inlet (D)
Hold the media valve in position while removing the four bolts screwed into
the valve from underneath the column (E).
Note:
Be aware that the residual liquid remaining in the media valve will spill out
when the bolts are removed.
2
Continue to hold the media valve in place while loosening the two locking
screws on the tube holder bolts (F).
3
The media valve and tube holder can now be removed downwards from
the column. Let the tube holder slide off its fastener. The following steps on
the media valve can be performed on a table.
4
Firmly press the release washer and hold it into the connector while removing
the pneumatic tube from pneumatic cylinder (1). Repeat the procedure to
disconnect the other pneumatic tube (2).
5
2
1
144 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.5 Media valve maintenance
ActionStep
Unscrew and remove the tube holder brackets (B) from the:6
• Slurry inlet tube,
• Bottom mobile phase tube, and
• Rinse inlet tube.
Let the pneumatic tubes stay connected to the tube holder (A).
A
BB B
Remove the tube holder and pneumatic tubes from the media valve.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 145
6 General maintenance and service
6.5 Media valve maintenance
ActionStep
Remove the three pipings and their O-rings from the media valve body:7
• Slurry inlet (A),
• Bottom mobile phase inlet/outlet (B),
• Rinse inlet (C).
Clean the O-rings with 20% ethanol. Replace with new O-rings if necessary.
A CB
For 300-600 columns equipped with plastic tubes, the rinse inlet has an inner
connector and additional O-ring.
146 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.5 Media valve maintenance
ActionStep
Unscrew the four bolts to remove the pneumatic cylinder and its piston from
the media valve outer body.
8
On AxiChrom 300-600 columns: When removing the piston, hold the upper
part of the pneumatic cylinder, to prevent the cylinder from falling apart.
Loosen the four bolts on the bottom of the media valve outer body. Do not
remove the bolts. Make sure a few millimeters of the bolt remain screwed
into the media valve. Press the bolts against a flat surface so that the bolts
push out the valve inner body, then remove the valve inner body.
9
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 147
6 General maintenance and service
6.5 Media valve maintenance
ActionStep
Remove and clean the O-ring from the valve outer body with 20% ethanol,
or replace with a new O-ring if necessary.
10
Remove and clean the four O-rings from the valve inner body with 20%
ethanol, or replace with new O-rings if necessary.
11
Assembling the media valve
At least two persons are needed when assembling the media valve.Note:
ActionStep
Refit all O-rings and the O-ring holder on the valve inner body.1
When refitting the diagonal O-ring on the valve inner body, make sure to
stretch it when putting it back in place. It must not be rolled into place.
148 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.5 Media valve maintenance
ActionStep
Refit the pneumatic cylinder into the media valve outer body.4
Make sure that the alignment of the pneumatic cylinder corresponds to the
image below. Tighten the bolts (crosswise) in steps to 1.7 Nm.
150 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.5 Media valve maintenance
ActionStep
Reposition the slurry inlet, rinse inlet and bottom mobile phase inlet/outlet
tubes on the media valve. Tighten the bolts enough to hold the tubes firmly
in position, but do not tighten the bolts fully at this stage.
5
CAUTION
The air from damaged pneumatic tubes may cause
injuries. Replace any damaged tubes.
A CB
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 151
6 General maintenance and service
6.5 Media valve maintenance
ActionStep
Place the media valve so that the mobile phase tube rests in the correct
position in the tube holder (A). Refit the tube holder bracket (B) on the mobile
phase tube and tighten the bolts slightly, so that the tube can still slide under
the bracket, to allow for adjustments in position when the tube holder is re-
fitted to the bottom backing plate.
6
A
BB B
Reposition the Slurry inlet tube (A) and Rinse inlet tube (C) against the media
valve body and tighten the tube bolts enough to hold the tubes firmly in
position, but do not tighten the bolts fully until verifying that the tubes have
been refitted at their correct angles.
Note:
To ensure that the Slurry inlet tube and the Rinse inlet tube are placed in their
correct angle when refitted, assemble the tube holder brackets, but tighten
the bolts only slightly, so that the tubes can still slide under the brackets.
7
A CB
152 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.5 Media valve maintenance
ActionStep
When all of the media valve tubes are correctly in position, tighten all of the
bolts holding the tubes to the media valve to 2.2 Nm.
8
For AxiChrom 1200-1600 columns: It is recommended to perform a media
valve leakage test at this stage, using the leakage test unit that is available
for this purpose (optional accessory).
Refit the two pneumatic tubes on the pneumatic cylinder according to the
image below. Make sure that the two tubes are connected to the correct
connectors.
9
2
1
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 153
6 General maintenance and service
6.5 Media valve maintenance
ActionStep
Re-assemble the media valve and tube holder under the column.10
F F
E
E
E
E
AB C D
FunctionPart
Valve pneumatic inletsA
Slurry inletB
Bottom mobile phase inlet/outletC
Rinse inletD
Media valve bolts screwed into the bottom backing plateE
Locking screws on the tube holder boltsF
1 Assemble the four media valve bolts into the bottom backing plate.
Tighten the bolts (crosswise) in steps up to 5 Nm.
2 Tighten the bolts on the tube holder brackets. The bolts should be tight-
ened evenly and not too hard, to avoid any damage to the tubes, espe-
cially if plastic tubes are used.
3 For media valves equipped with plastic tubes, make sure the tubes are
at a slight angle to the horizontal so that liquid in the tubes drains away
from the media valve.
4 Tightening the locking screws on the tube holder bolts to 10 Nm.
Connect the air supply tubes to the two valve pneumatic inlets on the tube
holder. Open the air supply to the column.
11
Connect the hoses to the Slurry inlet, Bottom mobile phase inlet/outlet , and
Rinse inlet. See Section 4.6 Connections, on page 68 for more information.
12
154 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.5 Media valve maintenance
ActionStep
Open the Luer connector on the tube of one of the flushing ports (for example,
flushing port A in the illustration of the locations of the flushing ports on
AxiChrom 1200-1600 columns).
1
A
D
B
C
Connect a syringe filled with 20% (v/v) ethanol to the Luer connector on the
tube of one of the two adjacent flushing ports (for example, port D).
2
Push fluid into the flushing port (port D) and through the flushing channel
until the fluid in the flushing channel has been replaced and fluid flows
steadily out from the first flushing port (port A).
3
Close flushing port D.4
Move around the column and open the Luer connector on the tube of the
next flushing port along (flushing port B).
5
Connect a syringe filled with 20% (v/v) ethanol to the Luer connector on the
tube of flushing port A.
6
156 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.6 Changing liquid in the flushing channels
6.7 Changing flushing connector O-rings
There are two flushing connectors for the 300-1000 columns and four flushing connectors
for the 1200-1600 columns, on the top of the adapter backing plate. Inside each flushing
connector are two O-rings, which should be changed at least once every two years. The
O-rings should be checked regularly and cleaned if needed.
Follow the instruction below to change the flushing connector O-rings.
ActionStep
Unscrew the flushing connector retaining nut. The image below is a cross-
section showing (1) the flushing connector retaining nut, (2) the flushing
connector, and the two O-rings indicated by arrows.
1
2
1
158 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
6 General maintenance and service
6.7 Changing flushing connector O-rings
7.2 Maintaining the adapter inlet/outlet
The instructions for adapter inlet/outlet (top mobile phase inlet/outlet) maintenance
applies only to AxiChrom 300-1000 columns. For AxiChrom 1200-1600 columns see
Section 8.2 Rinse nozzle maintenance, on page 207.
Adapter inlet/outlet maintenance is performed in connection with bed support mainte-
nance.
• For columns with stainless steel bed supports, see Section 7.3 Maintaining columns
with stainless steel bed supports, on page 166.
• For columns with plastic bed supports, see Section 7.4 Maintaining columns with
plastic bed supports, on page 181.
This section describes how to change the O-rings in the inlet/outlet valve.
DescriptionPart
Outer countersunk boltsA
Inner boltsB
ConnectorC
Valve outer bodyD
Plastic spacer and O-ring (only for 300, 400,
450 and 800 column sizes)
E
Valve inner bodyF
Valve body O-rings
G
A
B
E
C
D
F
G
G
G
G
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 163
7 Maintenance of 300-1000 columns
7.2 Maintaining the adapter inlet/outlet
7.3.1 Disassembling the bottom assembly
Removing the bed support
ActionStep
Make sure that the media valve is open. The media valve is open when the
piston in the center of the bed support is lowered. If the media valve is closed,
it can be opened using the Manual Control wizard in AxiChrom 300-1600
Master.
1
Use the bed support tool to remove the bed support screw in the center.2
NOTICE
Be careful not to slip with the bed support tool since
this may damage the bed support screw and/or the
bed support.
NOTICE
Be careful not to damage the threads when removing
the bed support screw.
Remove the O-ring on the bed support screw. Clean the O-ring with 20%
ethanol or replace it with a new O-ring if necessary.
3
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7 Maintenance of 300-1000 columns
7.3 Maintaining columns with stainless steel bed supports
7.3.1 Disassembling the bottom assembly
ActionStep
Unscrew the nuts underneath the bottom backing plate so that the bottom
bed support can be lifted. Screw the eye bolts (A) delivered with the column
into the holes and use them to lift the bed support by hand. The number of
eye bolts differs depending on the column size.
4
A
A
B
Remove the large O-ring (B) from the outer edge of the bed support. Clean
the O-ring with 20% ethanol or replace it with a new O-ring if necessary.
5
Remove the two small O-rings from center of the bed support.
Tip:
The two small O-rings in the center of the bed support are located one on
each side of the bed support. The lower O-ring is under the bed support and
must be removed after the bed support has been lifted off from the bottom
assembly.
6
Clean the O-rings with 20% ethanol or replace them with new O-rings if
necessary.
Remove the O-ring from the distributor plate. Clean the O-ring with 20%
ethanol or replace it with a new O-ring if necessary.
7
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 169
7 Maintenance of 300-1000 columns
7.3 Maintaining columns with stainless steel bed supports
7.3.1 Disassembling the bottom assembly
7.3.2 Disassembling the adapter
At least two persons are needed when disassembling the adapter assembly.Note:
Removing the bed support
ActionStep
Use the bed support tool to remove the bed support screw from the center
of the bed support. Hold the bed support tool vertically when removing the
bed support screw.
1
NOTICE
Be careful not to damage the threads when removing
the bed support screw.
170 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
7 Maintenance of 300-1000 columns
7.3 Maintaining columns with stainless steel bed supports
7.3.2 Disassembling the adapter
ActionStep
With the weight of the bed support assembly supported, unscrew the nuts
and remove the bed support assembly.
Tip:
Leave a couple of the nuts on opposite sides partly unscrewed. This will de-
crease the risk that the bed support assembly falls down by accident.
2
With the weight supported and at the same time gently pressing on the
partly unscrewed nuts might help to remove the bed support assembly if
stuck.
WARNING
The bed support and distributor assemblies are heavy,
particularly on larger columns. Make sure that these
components are securely supported and cannot fall
when fastening nuts or bolts are removed.
Note:
The nuts should release from the fasteners, leaving the fasteners attached to
the bed support. If any fastener releases from the bed support, refit the fas-
tener and tighten to a torque of 20 Nm.
Place the bed support on a clean, protective surface.3
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 171
7 Maintenance of 300-1000 columns
7.3 Maintaining columns with stainless steel bed supports
7.3.2 Disassembling the adapter
ActionStep
Remove the O-ring in the center of the top side of the bed support. Clean
the O-ring with 20% ethanol or replace it with a new O-ring if necessary.
4
Remove the scraper seal and its underlying O-ring from the bed support.5
Remove the O-ring in the center of the bottom side of the bed support.
Clean the scraper seal and O-rings with 20% ethanol or replace them with
new seal and O-rings if necessary.
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7 Maintenance of 300-1000 columns
7.3 Maintaining columns with stainless steel bed supports
7.3.2 Disassembling the adapter
ActionStep
Remove the O-ring from the distributor plate. Clean the O-ring with 20%
ethanol or replace it with a new O-ring if necessary.
6
Removing the adapter
inlet/outlet
ActionStep
Remove the adapter inlet/outlet from the column by unscrewing the four
outer countersunk bolts.
Note:
The 300, 400, 450, and 800 columns have a plastic spacer placed between
the adapter inlet/outlet and the column (indicated by arrows in the illustration).
Remove this plastic spacer and the O-ring underneath. Clean the O-ring with
20% ethanol or replace it with a new O-ring if necessary.
1
Replace the O-rings in the inlet/outlet valve if necessary (see Section 7.2
Maintaining the adapter inlet/outlet, on page 163).
2
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 173
7 Maintenance of 300-1000 columns
7.3 Maintaining columns with stainless steel bed supports
7.3.2 Disassembling the adapter
Removing the distributor plate
ActionStep
Remove the two flushing connectors according to the instruction in Sec-
tion 6.7 Changing flushing connector O-rings, on page158. Clean their O-rings
with 20% ethanol or replace them with new O-rings if necessary.
1
Remove the four bolts underneath the inlet/outlet.2
With the weight of the distributor plate supported, unscrew the bolts and
remove the distributor plate. The number of bolts varies with column size.
3
WARNING
The bed support and distributor assemblies are heavy,
particularly on larger columns. Make sure that these
components are securely supported and cannot fall
when fastening nuts or bolts are removed.
Remove the scraper seal and the large O-ring around the distributor plate.
Inspect for damage. Clean the scraper seal and O-ring with 20% ethanol or
replace them if necessary.
4
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7 Maintenance of 300-1000 columns
7.3 Maintaining columns with stainless steel bed supports
7.3.2 Disassembling the adapter
7.3.3 Assembling the adapter
At least two persons are needed when assembling the adapter.Note:
Refitting the distributor plate
ActionStep
Wet the large O-ring and scraper seal with 20% ethanol, then refit the O-
ring on the distributor plate and place the scraper seal on top of the O-ring.
1
Refit the distributor plate. Ensure that the holes in the distributor plate are
aligned with the flushing connectors as illustrated below. First, refit all bolts
finger-tight on the distributor plate. Then use a torque wrench to tighten the
M12 bolts on the distributor plate to 15 Nm and the four bolts underneath
the inlet/outlet assembly to 11 Nm.
2
Refit the two flushing connectors according to the instructions in Section 6.7
Changing flushing connector O-rings, on page 158.
3
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 175
7 Maintenance of 300-1000 columns
7.3 Maintaining columns with stainless steel bed supports
7.3.3 Assembling the adapter
Refitting the inlet/outlet
ActionStep
Refit the plastic spacer and O-ring (if used) on the inlet/outlet. The plastic
spacer is used on the 300, 400, 450 and 800 columns, and not on the 600
and 1000 columns. In the image below, arrows indicate the plastic spacer
and O-ring.
1
Refit the adapter inlet/outlet to the column. Make sure that the O-rings are
positioned correctly. Refit the inlet/outlet so that the tubing is closest to the
tube roller assembly. Tighten the four bolts crosswise to 5 Nm.
2
NOTICE
When fitting an old inlet/outlet on a newer column,
check if there are more than four threaded holes that
the inlet/outlet can be fitted to on top of the adapter.
If so, remove the guide pin from the inlet/outlet if it
exists.
Guide pin
176 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
7 Maintenance of 300-1000 columns
7.3 Maintaining columns with stainless steel bed supports
7.3.3 Assembling the adapter
Refitting the bed support
ActionStep
Refit the two center O-rings, one on each side of the bed support.1
Refit the bed support O-ring and scraper seal.2
While holding the bed support in place, refit the bed support fastening nuts,
and their associated plastic and stainless steel washers, so that the bed
support is retained. Do not tighten the nuts yet.
3
Wet the threads of the bed support tool, and then use the threaded side of
the bed support tool to center the bed support on the distributor plate. Make
sure that the center of the bed support exactly fits into the corresponding
countersink in the distributor plate, then tighten the bed support fastening
nuts fingertight, while the bed support tool is still in place.
4
Tighten the bed support fastening nuts using a torque wrench to tighten all
of these nuts crosswise to a torque of 10 Nm for 300-600 columns, or 15
Nm for 800 and 1000 columns.
5
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 177
7 Maintenance of 300-1000 columns
7.3 Maintaining columns with stainless steel bed supports
7.3.3 Assembling the adapter
ActionStep
Remove the bed support tool, wet surfaces with 20% ethanol and refit the
bed support screw. Carefully start to tighten the bed support screw by hand
making sure that the threads are not crossed. Finally tighten the bed support
screw firmly using the bed support tool. Make sure the bed support is secured
by the bed support screw.
6
NOTICE
Be careful not to damage the threads when refitting
the bed support screw.
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7 Maintenance of 300-1000 columns
7.3 Maintaining columns with stainless steel bed supports
7.3.3 Assembling the adapter
7.3.4 Assembling the bottom assembly
ActionStep
Refit the O-ring on the distributor plate.1
Refit the O-ring on the bed support (B).2
A
A
B
Refit the two small O-rings on the center of the bed support, one on each
side.
3
Refit the bed support on the distributor plate. Wet the threads of the bed
support tool, and then use the threaded side of the bed support tool to fit
and center the bed support on the distributor plate. Ensure that the center
of the bed support fits exactly into the corresponding countersink in the
distributor plate.
4
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 179
7 Maintenance of 300-1000 columns
7.3 Maintaining columns with stainless steel bed supports
7.3.4 Assembling the bottom assembly
ActionStep
Leave the bed support tool in place while you refit the nuts under the backing
plate. Tighten the nuts crosswise and in steps using a torque wrench, to 10
Nm torque for the 300-600 columns and 15 Nm for the 800 and 1000
columns.
Note:
The bed support fastening nuts can loosen when the tube is fastened into
place. Check torque tensions and retighten to 10 Nm for 300-600 columns,
or 15 Nm for 800-1000 columns, as required.
5
Remove the bed support tool, wet surfaces with 20% ethanol and refit the
bed support screw. Carefully start to tighten the bed support screw by hand
making sure that the threads are not crossed. Finally tighten the bed support
screw firmly using the bed support tool.
6
Make sure the bed support is secured by the bed support screw.
NOTICE
Be careful not to damage the threads when refitting
the bed support screw.
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7 Maintenance of 300-1000 columns
7.3 Maintaining columns with stainless steel bed supports
7.3.4 Assembling the bottom assembly
7.4.1 Disassembling the bottom assembly
ActionStep
Make sure that the media valve is open. The media valve is open when the
piston in the center of the bed support is lowered. If the media valve is closed,
it can be opened using the Manual Control wizard in AxiChrom 300-1600
Master.
1
Use the bed support tool to remove the bed support screw in the center.2
NOTICE
Be careful not to damage the threads when removing
the bed support screw.
Remove the O-ring on the bed support screw.3
Clean the O-ring with 20% ethanol or replace it with a new O-ring if neces-
sary.
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7 Maintenance of 300-1000 columns
7.4 Maintaining columns with plastic bed supports
7.4.1 Disassembling the bottom assembly
7.4.2 Disassembling the adapter
At least two persons are needed when disassembling the distributor plate.Note:
Removing the adapter
inlet/outlet
ActionStep
Remove the adapter inlet/outlet from the column by unscrewing the four
outer countersunk bolts. Arrows in the image below indicate the plastic
spacer and O-ring on 300, 400, 450 and 800 columns.
1
The 300, 400, 450, and 800 columns have a plastic spacer placed between
the adapter inlet/outlet and the column. Remove this plastic spacer and the
O-ring underneath. Clean the O-ring with 20% ethanol or replace it with a
new O-ring if necessary.
2
Replace the O-rings in the inlet/outlet valve if necessary (see Section 7.2
Maintaining the adapter inlet/outlet, on page 163).
3
Removing the bed support
assembly
ActionStep
Remove the two flushing connectors according to the instruction in Sec-
tion 6.7 Changing flushing connector O-rings, on page158. Clean their O-rings
with 20% ethanol or replace them with new O-rings if necessary.
1
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 185
7 Maintenance of 300-1000 columns
7.4 Maintaining columns with plastic bed supports
7.4.2 Disassembling the adapter
ActionStep
Remove the four bolts underneath the inlet/outlet.2
Remove the bolts on the top of the distributor plate. The number of bolts
varies with columns size.
3
186 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
7 Maintenance of 300-1000 columns
7.4 Maintaining columns with plastic bed supports
7.4.2 Disassembling the adapter
ActionStep
With the weight of the bed support assembly supported, unscrew the nuts
and remove the bed support assembly.
Tip:
Leave a couple of the nuts on opposite sides partly unscrewed. This will de-
crease the risk that the bed support assembly falls down by accident.
4
WARNING
The bed support and distributor assemblies are heavy,
particularly on larger columns. Make sure that these
components are securely supported and cannot fall
when fastening nuts or bolts are removed.
Note:
The nuts should release from the fasteners, leaving the fasteners attached to
the distributor plate. If any fastener releases from the distributor plate instead,
refit the fastener and tighten to a torque of 2 Nm.
Place the bed support and distributor assembly on a clean firm surface, al-
lowing it to rest on the fasteners.
5
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 187
7 Maintenance of 300-1000 columns
7.4 Maintaining columns with plastic bed supports
7.4.2 Disassembling the adapter
ActionStep
Remove the upper scraper seal and O-ring. Clean the seal and O-ring with
20% ethanol or replace with new components if necessary.
4
Remove the screws holding the distributor ring using an Allen key. Hexagon
socket screws holding the distributor ring are illustrated below.
5
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 189
7 Maintenance of 300-1000 columns
7.4 Maintaining columns with plastic bed supports
7.4.2 Disassembling the adapter
ActionStep
Remove the distributor ring from the distributor.6
Remove the lower scraper seal and O-ring. Clean the seal and O-ring with
20% ethanol or replace with new components if necessary.
7
190 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
7 Maintenance of 300-1000 columns
7.4 Maintaining columns with plastic bed supports
7.4.2 Disassembling the adapter
7.4.3 Assembling the adapter
At least two persons are needed when assembling the adapter.Note:
Reassembling the bed support
assembly
ActionStep
Wet the O-ring and lower scraper seal with 20% ethanol. With the ribbed
side of the distributor facing downwards, refit the O-ring on the distributor
plate and place the scraper seal on top of the O-ring.
1
Place the distributor ring adapter on the distributor. The two steering pins
ensure that the ring can only be placed in one orientation.
2
Refit the screws and washers to secure the distributor ring adapter. Tighten
the screws with a torque wrench to 4 Nm. Hexagon socket screws holding
the distributor ring are illustrated below.
3
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 191
7 Maintenance of 300-1000 columns
7.4 Maintaining columns with plastic bed supports
7.4.3 Assembling the adapter
ActionStep
Turn the distributor over so that it rests on the fasteners. Make sure that the
lower scraper seal and O-ring remain in place.
4
Place the new bed support on the distributor. The smoother side of the bed
support shall face the interior of the column.
5
Refit the O-ring in the bed support screw, then wet the O-ring and the bed
support screw with 20% ethanol. Use the removal tool to help fit the O-ring
if necessary.
6
Refit the bed support screw. Carefully start to tighten the bed support screw
by hand making sure that the threads are not crossed. Finally tighten the
bed support screw more firmly using the bed support tool. Make sure the
bed support is secured by the bed support screw.
7
NOTICE
Be careful not to damage the threads when refitting
the bed support screw.
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7 Maintenance of 300-1000 columns
7.4 Maintaining columns with plastic bed supports
7.4.3 Assembling the adapter
ActionStep
Refit the snap ring, tapping gently with the plastic mallet if necessary. Use
the groove on the mallet head to lever the snap ring over the edge of the
bed support if the fit is tight.
8
Note:
Check that the snap ring is correctly and evenly mounted around the whole
circumference of the bed support.
The illustration shows a cross-section of a snap-ring mounted correctly and
two images of a snap-ring mounted incorrectly.
A
BC
D
FunctionPart
Snap ringA
Scraper sealB
DistributorC
Bed supportD
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 193
7 Maintenance of 300-1000 columns
7.4 Maintaining columns with plastic bed supports
7.4.3 Assembling the adapter
Refitting the bed support
assembly
ActionStep
Wet the O-ring and upper scraper seal with 20% ethanol. Refit the O-ring
on the distributor and place the scraper seal on top of the O-ring.
1
With the bed support and distributor assembly supported, refit the assembly
on the backing plate. Position the assembly so that the holes in the assembly
and backing plate are aligned (the assembly can only be mounted in one
orientation on the backing plate). Fit both stainless steel and plastic washers
onto each of the threaded bed support fasteners, with the plastic washer
closest to the backing plate. Tighten all of the bed support fastening nuts
finger-tight, then use a torque wrench to tighten all of these nuts crosswise
to a torque of 4 Nm.
2
194 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
7 Maintenance of 300-1000 columns
7.4 Maintaining columns with plastic bed supports
7.4.3 Assembling the adapter
ActionStep
Refit the bolts on top of the distributor plate. Fit both stainless steel and
plastic washers on each screw, with the plastic washer closest to the backing
plate. Tighten the bolts to 15 Nm.
3
A
B
FunctionPart
Stainless steel washerA
Plastic washerB
Refit the four bolts underneath the inlet/outlet assembly and tighten to
11 Nm.
4
Refit the two flushing connectors according to the assembly instructions in
Section 6.7 Changing flushing connector O-rings, on page 158.
5
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 195
7 Maintenance of 300-1000 columns
7.4 Maintaining columns with plastic bed supports
7.4.3 Assembling the adapter
Refitting the adapter inlet/outlet
ActionStep
Refit the plastic spacer and O-ring (if used) on the inlet/outlet. The plastic
spacer is used on the 300, 400, 450 and 800 columns, but not on the 600
and 1000 columns. Arrows in the illustration below indicate the plastic
spacer and O-ring.
1
Refit the adapter inlet/outlet to the column. Make sure that the O-rings are
positioned correctly. Refit the inlet/outlet so that the tubing is closest to the
tube roller assembly. Tighten the four bolts cross-wise to 5 Nm.
2
NOTICE
When fitting an old inlet/outlet on a newer column, check if there
are more than four threaded holes that the inlet/outlet can be fitted
to on top of the adapter. If so, remove the guide pin from the in-
let/outlet if it exists.
Guide pin
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7 Maintenance of 300-1000 columns
7.4 Maintaining columns with plastic bed supports
7.4.3 Assembling the adapter
8.1 Maintenance tools for 1200-1600 columns
Introduction
This section describes tools that are used specifically when performing service and
maintenance on AxiChrom 1200-1600 columns. General tools used with all column sizes
are described in Section 6.2 General maintenance tools, on page 111.
NOTICE
Always use appropriate tools and the correct tightening torques
for bolts when working with the AxiChrom column and AxiChrom
Master unit. Only the special toolkit provided by GE for the AxiChrom
column should be used.
Bed support tool
The bed support tool is used for removing and replacing the bed support screw. Engage
the pins on the tool firmly in the screw when using the tool.
The bed support tool for 1200-1600 columns is shown below.
The threaded end of the tool is used with stainless steel bed supports to assist in centering
the bed support on the distributor during assembly.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 199
8 Maintenance of 1200-1600 columns
8.1 Maintenance tools for 1200-1600 columns
Bed support nut tool
The bed support nut tool is used for tightening the bed support nuts during assembly
and disassembly of the plastic bed supports on 1200-1600 columns.
Bed support lifting device
The bed support lifting device is used for the disassembly and assembly of AxiChrom
1200-1600 columns.
The bed support lifting device is used for example during:
• removal/fitting of plastic and stainless steel adapter bed supports, and the adapter
distributor plate,
• removal/fitting of stainless steel bottom bed supports, and the bottom distributor
plate.
The maximum lifting capacity of the bed support lifting device is 120 kg.
If a bed support lifting device is not used when performing the disassembly
and assembly of AxiChrom 1200-1600 columns, it is recommended that a
minimumof five people are on hand towork together to performmanual lifting
operations. As it is strongly recommended that a lifting device is used to per-
form maintenance steps, manual lifting operations are not described.
Note:
200 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.1 Maintenance tools for 1200-1600 columns
Illustration of the lifting device
1
5
6
3
10
11
4
2
9
8
7
NoteDescriptionPart
The lifting device has three, evenly
spaced, extendable arms.
Tri-arm (3×)1
Each extendable tri-arm has length ad-
justment locking holes for the length ad-
justment screw (1200, 1400 and 1600
extendable positions).
Tri-arm length adjustment
screw (one on each arm)
2
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 201
8 Maintenance of 1200-1600 columns
8.1 Maintenance tools for 1200-1600 columns
NoteDescriptionPart
The upper and lower clamps are screwed
onto the threaded attachment at the end
of each tri-arm as required, according to
the procedure performed.
Upper (a) and lower (b) remov-
able clamps1
a
b
3
The counter nuts fix the threaded screw
in place on the tri-arm fittings. The
counter nuts are loosened to adjust the
threaded screw upwards or downwards
and when using the threaded screws to
lift stainless steel bottom bed supports
and corresponding distributor plates.
Counter nuts (a) on the tri-arm
threaded screw attachment
(b)
4
Note:
The image shows the threaded
screw attachment with the
lower clamp fitted.
Used for columns with plastic bed sup-
ports and distributors, and for the distrib-
utor of columns with stainless steel bed
supports.
Tri-arm center fitting – type 15
Used only with stainless steel bed sup-
ports.
Tri-arm center fitting – type 26
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8 Maintenance of 1200-1600 columns
8.1 Maintenance tools for 1200-1600 columns
NoteDescriptionPart
Used with tri-arm center fitting type 2 to
securely fixing stainless steel bed sup-
ports to the bed support lifting device
before moving the lifting device away
from the column or rotating the tri-arms.
Center fitting lock and pin7
The stabilizing screws are tightened to
fix the individual tri-arms in place and
loosened to make minor adjustments to
the positioning of the tri-arms.
Stabilizing screws (3×)8
Used to rotate the angle of the tri-arms.Side winch9
Used to raise and lower the tri-arms.Rear winch10
Used to make minor position adjustments
to level the tri-arms.
Jacks on back wheels (2×)11
1 The threaded screw attachment is used in different ways to perform different lifting and moving
functions. For example:
• with no upper or lower clamps fitted, to lift stainless steel bottom bed supports.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 203
8 Maintenance of 1200-1600 columns
8.1 Maintenance tools for 1200-1600 columns
• with the lower clamp fitted, to support stainless steel and plastic adapter bed supports.
• with both the upper and lower clamps fitted to secure bed supports and distributor plates
to the tri-arms during movement of the lifting device and rotation of the tri-arms.
For further information about the bed support lifting device, refer to the third party user
documentation provided by the manufacturer.
WARNING
Follow the instructions provided by the manufacturer and all addi-
tional user documentation safety instructions when using the lifting
device.
WARNING
Make sure the lifting device is in perfect working order before use.
If any damage is suspected, do not use the lifting device.
204 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.1 Maintenance tools for 1200-1600 columns
Bed support protection
A bed support protection mat and disposable protective is provided with the column.
These are also recommended accessories to keep on site for use during the maintenance
of AxiChrom 1200-1600 columns to protect the bottom bed support.
The bed protection consists of two layers:
• a thin disposable plastic film (sold in sets of five) that is placed directly onto the bed
support
• a thicker reusable mat that is placed on top of the plastic film.
The mats can be ordered from GE for the appropriate column size.
Nozzle O-ring assembly tool
The replacement of rinse nozzle tip O-rings is performed by manually removing the O-
rings from the respective nozzle tip grooves, cleaning the grooves and fitting new O-
rings.
A conical nozzle O-ring expansion tool is available for expanding and fitting the new O-
rings into place on the nozzle tip during rinse nozzle assembly, see Assembling the rinse
nozzle, on page 212.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 205
8 Maintenance of 1200-1600 columns
8.1 Maintenance tools for 1200-1600 columns
Rinse nozzle parts
9
22
1
10
11
12
13
14
15
16
17
8
7
6
5
4
3
2
21
18
19
20
23
208 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.2 Rinse nozzle maintenance
A
B
To switch between nozzle tube positions the tube holder is simply unscrewed and rotated
180 degrees. The following image shows the lower tube position (A), used when columns
are fitted with plastic bed supports. The arrow indicates how the position of the nozzle
tube holder must be rotated through 180 degrees to position (B) to use the nozzle in
columns with stainless steel bed supports.
Disassembling the rinse nozzle
The numerical designations in the following instructions refer to the schematic diagram
in Rinse nozzle parts, on page 208.
210 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.2 Rinse nozzle maintenance
ActionStep
Recommended: If the media valve is not already in the Open position, set
the media valve to Open using the Manual Control wizard in AxiChrom
300-1600 Master.
1
Make sure that all hoses have been disconnected to the:
• slurry inlet,
• bottom mobile phase inlet/outlet,
• rinse inlet, and
• pneumatic inlets.
NOTICE
If the column is equipped with a bypass valve, make
sure that the position of the bypass valve will not re-
strict the movement of the bottom backing plate.
Make sure that the column disassembly wizard steps have been followed
and that the column tube is locked in the swing out position before beginning
to follow the Media valve access wizard procedures. In the Maintenance
menu selectMedia valve access and then selectDisassembly. The adapter
will move down in the next step.
2
Press Confirm.
216 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.3 Lifting and lowering the bottom backing plate
ActionStep
Carefully position the four lifting blocks over the guide pins, as shown in the
image.
3
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 217
8 Maintenance of 1200-1600 columns
8.3 Lifting and lowering the bottom backing plate
ActionStep
Insert the bottom flange bolts and washers that were removed before swing
out of the column tube, as shown in the image. Fit also the appropriate dis-
tance spacers under the heads of the flange bolts as shown in the following
table.
4
Stainless steel tubeAcrylic tubeColumn size
SpacerSpacer1200
SpacerNo spacer1400
SpacerSpacer1600
When the bolts and spacers, if applicable, are in place, press Confirm.
NOTICE
When bottom flange bolt distance spacers are required
they are provided with the column. The function of the
spacers is to prevent the bottom flange bolts from
coming into contact with the column stand when lifting
and lowering the bottom backing plate. The spacers
are fitted under the heads of the bottom flange bolts
when the bolts are used to attach the lifting blocks.
218 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.3 Lifting and lowering the bottom backing plate
ActionStep
Attach the washers and nuts to the bolts to fasten the lifting blocks to the
bottom backing plate. Finger tighten the bolts, then tighten all four lifting
block bolts to 180 Nm.
5
Press Confirm.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 219
8 Maintenance of 1200-1600 columns
8.3 Lifting and lowering the bottom backing plate
ActionStep
Remove the three nuts shown in the image from the bottom of the column
stand.
6
NOTICE
Before raising the adapter, make sure that all nuts have
been removed. If a nut is forgotten the column and
related parts can be damaged.
Press Confirm.
220 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.3 Lifting and lowering the bottom backing plate
ActionStep
The adapter is automatically raised, hoisting the bottom plate. Make sure
there are no obstacles in the way of the bottom plate.
7
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8 Maintenance of 1200-1600 columns
8.3 Lifting and lowering the bottom backing plate
ActionStep
Screw the three safety rods onto the three threaded pins in the positions
shown in the image. Make sure that the safety rods are fully screwed onto
the threaded pins.
8
The adapter will move down in the next step. Make sure there are no obsta-
cles or body parts between the safety rods and the column stand.
Press Confirm.
222 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.3 Lifting and lowering the bottom backing plate
ActionStep
Adjust each safety rod position by screwing the rods down until each rod
sits firmly into its socket in the column stand.
9
Press Confirm.
Continue to Disassembling the media valve, on page 142 and subsequently
Assembling the media valve, on page 148 to do the maintenance work.
10
Lowering the bottom backing
plate
After media valve maintenance has been completed, lower the bottom backing plate
according to the following instructions.
WARNING
Follow all of the user documentation safety instructions and the
instructions provided by the AxiChrom Master when performing
maintenance on the column.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 223
8 Maintenance of 1200-1600 columns
8.3 Lifting and lowering the bottom backing plate
ActionStep
In theMaintenancemenu selectMedia valve accessand then selectAssem-
bly. Make sure that all of the lifting blocks are firmly in place, and then press
Continue.
1
Result: The adapter will move up in the next step.
Unscrew and remove the three safety rods shown in the image, and then
wrap PTFE tape around the threads of each of the three pins.
2
Press Confirm.
Make sure that there are no obstacles near the column that could prevent
the threaded pins from entering the corresponding holes in the bottom of
the column stand.
3
Press Confirm. The adapter will move down in the next step.
224 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.3 Lifting and lowering the bottom backing plate
ActionStep
Attach the three fastening nuts and tighten to 80 Nm.4
Press Confirm.
Carefully loosen and remove the nuts and washers from the lifting block
bolts.
5
Press Confirm.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 225
8 Maintenance of 1200-1600 columns
8.3 Lifting and lowering the bottom backing plate
ActionStep
Carefully remove the bolts and washers from each of the four lifting blocks.6
Press Confirm.
NOTICE
Remove also the bottom flange bolt distance spacers.
This does not apply for AxiChrom 1400 columns with
acrylic tubes, as no distance spacers are used with
these columns.
Carefully remove all four lifting blocks. The adapter will move up in the next
step.
7
Press Confirm.
The adapter is automatically raised to the top position. Media valve Access
– Assembly is completed.
8
Press Continue to return to the Maintenance menu.
The column is now ready for column assembly. See the Column assembly
wizard, on page 128 for more information.
9
226 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.3 Lifting and lowering the bottom backing plate
8.4.1 Disassembling the bottom assembly
Removing the bed support
ActionStep
Make sure the media valve is open. The media valve is open when the piston
in the center of the bed support is lowered. If the media valve is closed, it
can be opened using theMANUALCONTROLwizard in the AxiChrom Master.
1
Use the bed support tool to remove the bed support screw in the center.2
NOTICE
Be careful not to slip with the bed support tool since
this may damage the bed support screw and/or the
bed support.
NOTICE
Be careful not to damage the threads when removing
the bed support screw.
Remove the O-ring on the bed support screw.3
Clean the O-ring with 20% ethanol or replace with a new O-ring, if necessary.
230 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.1 Disassembling the bottom assembly
ActionStep
Remove the large O-ring from the outer edge of the bed support. Clean the
O-ring with 20% ethanol or replace it with a new O-ring if necessary.
4
Remove the small O-ring from center of the bed support.
Note:
There are two small O-rings in the center of the bed support, located one on
each side of the bed support. The lower O-ring (underneath the center of the
bed support) must be removed after the bed support has been lifted off from
the distributor plate.
5
Unscrew the bed support fastening nuts from around the circumference of
the column, underneath the bottom backing plate.
6
Make sure that the bed support lifting device is equipped with the bed support
fitting (see Illustration of the lifting device, on page 201, item 6) and the
threaded screws. If this is not the case, remove the clamps and install the
threaded screw fittings as shown in the illustration, and screw down the
counter nuts to hold the threaded screw fittings in place. Refer toBed support
lifting device, on page 200.
7
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8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.1 Disassembling the bottom assembly
ActionStep
Loosen the stabilizing screws in the center of the tri-arms of the bed support
lifting device. This will make it easier to correctly fasten the tri-arms to the
bed support. Refer to Bed support lifting device, on page200 for more infor-
mation.
8
Wheel the bed support lifting device into position, a few centimeters above
the bed support.
Note:
To correctly align the tri-arm screw fittings of the bed support lifting device
with the corresponding threaded holes in the bed support or distributor plate
of the bottom columnassembly, the bed support lifting devicemust bemoved
into position from the side of the column as shown in the following illustration.
9
Align the center of the tri-arm with the center of the bottom bed support.10
Check that the tri-arm threaded screws are aligned with their corresponding
threaded holes (M10) in the bed support, but make sure that the tri-arm
screws are retracted so that it is the center fitting that first comes into con-
tact with the bed support when the bed support lifting device is lowered.
11
Lower the bed support lifting device tri-arm, using the rear winch on the
lifting device, until the bed support fitting on the lifting device enters and
rests against the center of the bed support and the tri-arms are levelly posi-
tioned above the bed support.
Tip:
If necessary, the jacks on the back wheels of the bed support lifting device
can be used to level the arms of the lifting device.
12
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8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.1 Disassembling the bottom assembly
ActionStep
Loosen the counter nuts of the tri-arm threaded screws.13
Make sure that the tri-arms are still levelly positioned above the bed support,
and then screw the tri-arm threaded screws firmly into the corresponding
threaded holes in the bed support.
Tip:
If necessary, the jacks on the back wheels of the bed support lifting device
can be used to level the arms of the lifting device.
14
Tighten the counter nuts on each of the tri-arm threaded screws, and then
tighten the stabilizing screws (illustrated below) at the center of the tri-arms.
15
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8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.1 Disassembling the bottom assembly
ActionStep
Use the rear winch on the lifting device to winch up the tri-arms and lift the
bed support until it is fully clear of the bottom distributor, and then fit the
center lock (see Illustration of the lifting device, on page201) before carefully
moving the bed support lifting device away from the column.
16
NOTICE
Make sure that the bed support is raised far enough
to prevent any possibility of the bed support fasteners
being able to scrape the polypropylene surface of the
distributor.
NOTICE
Take care not to bump the bed support into the column
stand.
Remove the lower O-ring in the center of the bed support. Clean the O-ring
with 20% ethanol or replace it with a new O-ring if necessary.
17
Move the bed support to desired position.18
Remove the O-ring from the distributor plate. Clean the O-ring with 20%
ethanol or replace it with a new O-ring if necessary.
19
234 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.1 Disassembling the bottom assembly
Removing the distributor plate
Before removing the distributor plate the bed supportmust already have been
removed separately.
Note:
ActionStep
Make sure that the bottom bed support has been removed, and then lift the
bottom backing plate and disassemble and remove the media valve as de-
scribed in Lifting the bottom backing plate, on page 215 and Disassembling
the media valve, on page 142.
1
Unscrew all of the remaining M12 bolts holding the distributor to the bottom
backing plate, including the four bolts located under the position previously
occupied by the media valve.
2
Lower the bottom backing plate as described in Lowering the bottombacking
plate, on page 223.
3
Wet the distributor plate fitting with 20% ethanol and then screw the distrib-
utor plate fitting into the distributor plate center, tightening slowly. Refer to
Bed support lifting device, on page 200.
4
Make sure that the bed support lifting device is equipped with the threaded
screws.
5
Loosen the stabilizing screws in the center of the tri-arms of the bed support
lifting device. This will make it easier to correctly fasten the tri-arms to the
distributor plate.
6
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 235
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.1 Disassembling the bottom assembly
ActionStep
Move the bed support lifting device into position over the distributor, with
the center of the lifting device aligned with the center of the distributor and
the tri-arms a few centimeters above the distributor plate.
7
Note:
To correctly align the tri-arm screw fittings of the bed support lifting device
with the corresponding threaded holes in the bed support or distributor plate
of the bottom columnassembly, the bed support lifting devicemust bemoved
into position from the side of the column as shown in the following illustration.
Align the three threaded screws on the bed support lifting device tri-arm
with the corresponding three threaded holes (M24) in the distributor.
8
236 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.1 Disassembling the bottom assembly
ActionStep
Lower the bed support lifting device tri-arm, using the rear winch. Make sure
that the distributor fitting is aligned to the tri-arm adapter mounted in the
center. Stop winching when the distributor fitting has clicked into place on
the tri-arm adapter.
Tip:
If necessary, the jacks on the back wheels of the bed support lifting device
can be used to level the lifting device.
9
Loosen the counter nuts of the tri-arm screws. Refer to Bed support lifting
device, on page 200.
10
Insert the threaded screws into the matching threaded holes on the distrib-
utor and fasten firmly.
11
Make sure that the tri-arms are levelly positioned above the distributor.12
Tighten the counter nuts on each of the tri-arm threaded screws, and then
tighten the stabilizing screws (illustrated below) at the center of the tri-arms.
13
Lift the distributor, making sure that it is well clear of the bottom backing
plate, and then slowly move the bed support lifting device away from the
column.
14
If applicable, clean the distributor plate or change distributor plate type.15
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 237
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.1 Disassembling the bottom assembly
8.4.2 Disassembling the adapter
NOTICE
During all maintenance on the adapter and rinse nozzle make sure
that the bottom bed support and the bottom distributor are pro-
tected, for example by covering them with a plastic film and a bed
protection mat.
Remove the rinse nozzle
If there is little clearance space available to maneuver the rinse nozzle, pres-
surized air can be used to blow the nozzle down into its open (lowest) position.
Tip:
ActionStep
Unscrew and remove the four outer nozzle bolts.1
238 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.2 Disassembling the adapter
ActionStep
Carefully lift the rinse nozzle (and spacer, if present) up and out from the
column, tilting the nozzle as required for clearance.
2
Tip:
Themobile phase inlet/outlet connection tube can be removed from the rinse
nozzle before removing the nozzle from the column. This will provide more
clearance space above the nozzle.
If necessary, disassemble the rinse nozzle and clean the O-rings in accor-
dance with the instructions in Section 8.2 Rinse nozzle maintenance, on
page 207.
3
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8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.2 Disassembling the adapter
Remove the bed support
ActionStep
Use the bed support tool to remove the bed support screw from the center
of the bed support. Hold the bed support tool vertically and firmly pressed
up against the bed support screw when removing the bed support screw.
1
NOTICE
Be careful not to slip with the bed support tool since
this may damage the bed support screw and/or the
bed support.
NOTICE
Be careful not to damage the threads when removing
the bed support screw.
Remove the O-ring in the center of the bottom side of the bed support.2
240 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.2 Disassembling the adapter
ActionStep
Make sure that the bed support lifting device is equipped with the center
fitting for stainless steel bed supports and the tri-arm lower clamps. Other-
wise install the clamps and the bed support fitting. (See Section 8.1 Mainte-
nance tools for 1200-1600 columns, on page199 for more information about
the clamps and bed support fittings.)
3
Loosen the stabilizing screws in the center of the tri-arms of the bed support
lifting device. This will make it easier to correctly fasten the tri-arms to the
bed support.
4
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 241
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.2 Disassembling the adapter
ActionStep
Move the bed support lifting device into position below the bed support so
that the middle of the tri-arm is directly under the middle of the bed support
and the tri-arm ends are under the outer rim of the bed support.
Note:
The bed support lifting device must be moved into position from the side of
the column, as shown in the illustration.
5
Use the rear winch to gently lift the tri-arm towards the adapter. Stop
winching when the bed support rests on the center fitting.
Note:
Make sure that the bed support fitting in themiddle of the tri-arm fits into the
center of the bed support andmakes contact with the bed support before the
tri-arm clamps. Retract the clamps a few turns if necessary.
6
Tip:
If necessary, the jacks on the back wheels of the bed support lifting device
can be used to level the lifting device.
242 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.2 Disassembling the adapter
ActionStep
Adjust the lower clamps up against the bed support, so that the bed support
is resting securely and evenly on the three clamps of the lifting device. Level
the tri-arm against the bed support.
7
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 243
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.2 Disassembling the adapter
ActionStep
Remove the bed support fastening nuts.8
WARNING
The bed support and distributor assemblies are heavy,
particularly on larger columns. Make sure that these
components are securely supported and cannot fall
when fastening nuts or bolts are removed.
Note:
The number of fastening nuts may vary from those shown in the illustration,
depending on column size. The bed support fastening nuts should release
from the fasteners, leaving the fasteners attached to the distributor plate. If
any fastener releases from the bed support ring instead, refit the fastener and
tighten to a torque of 20 Nm.
244 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.2 Disassembling the adapter
ActionStep
Tighten the stabilizing screws in the center of the tri-arms on the bed support
lifting device, and then slowly lower the tri-arm, making sure that the bed
support separates from the distributor all the way around the circumference
of the column.
9
Lower the bed support lifting device until the bed support is fully clear of the
distributor.
10
NOTICE
Make sure that the bed support is lowered far enough
to prevent any possibility of the bed support fasteners
being able to scrape the polypropylene surface of the
distributor.
Carefully move the bed support lifting device away from the column, taking
care not to bump the bed support into the column stand.
11
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 245
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.2 Disassembling the adapter
ActionStep
Remove the outer O-ring from the bed support. Clean the O-ring with 20%
ethanol or replace it with a new O-ring if necessary.
12
Remove the scraper seal and its underlying O-ring from the bed support.13
Clean the scraper seal and O-rings with 20% ethanol or replace them with
new seal and O-rings if necessary.
246 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.2 Disassembling the adapter
ActionStep
Remove the O-ring in the center of the top side of the bed support. Clean
the O-ring with 20% ethanol or replace it with a new O-ring if necessary.
14
Fit the center lock to the fitting in the center of the tri-arms if the bed support
lifting device is to be used to maneuver the bed support away from the area
of the column, so that the bed support can be removed from the lifting device.
15
Remove the O-ring from the distributor plate. Clean the O-ring with 20%
ethanol or replace it with a new O-ring if necessary.
16
Remove the distributor plate
ActionStep
Remove the four flushing connectors according to the instruction in Sec-
tion 6.7 Changing flushing connector O-rings, on page158. Clean their O-rings
with 20% ethanol or replace them with new O-rings if necessary.
1
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 247
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.2 Disassembling the adapter
ActionStep
Remove the four bolts underneath the rinse nozzle.2
Fasten the distributor plate fitting into the distributor plate center.3
Make sure that the bed support lifting device is equipped with the lower
clamps. Otherwise install the clamps in accordance with the image above.
Note:
For description and specifications for the bed support lifting device see Bed
support lifting device, on page 200.
4
Loosen the stabilizing screws in the center of the tri-arms of the bed support
lifting device. This will make it easier to correctly fasten the tri-arms to the
distributor plate.
5
248 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.2 Disassembling the adapter
ActionStep
Move the bed support lifting device into position below the distributor plate
so that the middle of the tri-arm is directly under the middle of the distributor
plate and the tri-arm ends are under the outer rim of the distributor plate.
6
Use the rear winch to gently lift the tri-arm towards the adapter. Make sure
that the distributor fitting is aligned with the tri-arm adapter. Stop winching
when the distributor fitting spring-loaded pin has clicked into place on the
tri-arm adapter and the distributor plate rests on the tri-arm.
Tip:
If necessary, the jacks on the back wheels of the bed support lifting device
can be used to level the lifting device.
7
Adjust the tri-arm lower clamp fittings until all three clamps sit tightly against
the distributor, with the tri-arms of the bed support lifting device level with
the distributor plate.
8
Unscrew the bolts to remove the distributor plate. The number of bolts varies
with column size.
9
WARNING
The bed support and distributor assemblies are heavy,
particularly on larger columns. Make sure that these
components are securely supported and cannot fall
when fastening nuts or bolts are removed.
Tighten the stabilizing screws in the center of the tri-arms of the bed support
lifting device.
10
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 249
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.2 Disassembling the adapter
ActionStep
Slowly lower the tri-arm a few centimeters, while making sure the distributor
plate separates from the adapter.
11
Lower the distributor until it is clear of the column, and then remove the
scraper seal and O-ring from the distributor plate.
12
Clean the scraper seal, the large O-ring around the distributor plate and the
O-ring groove with 20% ethanol.
Screw the upper clamps onto the tri-arm fittings, to clamp the distributor
plate securely in place on the bed support lifting device, and then pull out
the bed support lifting device slowly, taking care not to bump the distributor
plate into the column stand.
13
Move the lifting device away from the column, and then rotate the tri-arms
of the lifting device through 180 degrees so that the distributor plate can
be carefully maneuvered onto a clean, protected surface and removed from
the bed support lifting device.
14
If applicable, clean the distributor plate or change the distributor plate type.15
For reassembly instructions, see Section 8.4.4 Assembling the bottomassembly,
on page 262.
Note:
250 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.2 Disassembling the adapter
8.4.3 Assembling the adapter
For description and specifications for the bed support lifting device see Bed
support lifting device, on page 200.
Note:
Refit the distributor plate
ActionStep
Make sure that the clean distributor plate is securely fastened to the bed
support lifting device using the upper tri-arm clamps and the distributor fit-
ting. Rotate the tri-arm of the lifting device if required, so that the distributor
plate is in the correct orientation for fitting to the adapter.
1
Move the bed support lifting device close to the column.2
Make sure that the lifting device is stationary, and then remove the upper
tri-arm clamps to allow the distributor plate O-ring and scraper seal to be
fitted without hindrance.
3
Wet the large O-ring and scraper seal with 20% ethanol, then refit the O-
ring on the distributor plate and place the scraper seal on top of the O-ring.
4
Move the bed support lifting device with the distributor plate into position
below the adapter backing plate, so that the middle of the tri-arm and dis-
tributor plate are directly under the middle of the adapter backing plate.
Take care not to bump the distributor plate into the column stand.
5
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8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.3 Assembling the adapter
ActionStep
Make sure that the holes for the flushing connectors in the distributor plate
are aligned with the corresponding holes in the backing plate, as illustrated
below.
6
Use the rear winch to gently lift the tri-arm and distributor plate towards
the backing plate. Make sure that the center of the distributor plate aligns
with the center of the backing plate. Stop winching when the distributor
plate touches the backing plate.
Tip:
If necessary, the jacks on the back wheels of the bed support lifting device
can be used to level the lifting device.
7
252 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.3 Assembling the adapter
ActionStep
Refit all of the distributor plate bolts and finger tighten, then use a torque
wrench to tighten the M12 bolts on the distributor plate to 15 Nm and the
four bolts underneath the rinse nozzle assembly to 11 Nm.
8
Release the distributor plate from the tri-arms of the lifting device using the
spring loaded pin on the center fitting and lower the tri-arm a few centimeters
using the rear side winch. Remove the distributor fitting from the center of
the distributor plate, then carefully move the bed support lifting device away
from the column.
9
Refit the flushing connectors according to the instructions in Section 6.7
Changing flushing connector O-rings, on page 158.
10
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8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.3 Assembling the adapter
ActionStep
Refit the bed support O-ring and scraper seal, and then then fit the thin
outer O-ring onto the rim of the bed support. Wet the O-rings with 20%
ethanol.
6
Tip:
To make installation easier, make sure that the thin O-ring is thoroughly
wetted with 20% ethanol during assembly. The thin O-ring may pop off if it
is dry during assembly.
Move the bed support lifting device with the bed support into position below
the distributor plate so that the middle of the tri-arm and bed support is di-
rectly under the middle of the distributor plate. Take care not to bump the
bed support into the column stand.
7
Make sure that the bed support fasteners are aligned with the corresponding
holes in the distributor plate.
8
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8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.3 Assembling the adapter
ActionStep
Use the rear winch to gently lift the tri-arm and bed support towards the
distributor plate. Make sure that the bed support is level in the lifting device
when raised. Use the stabilizing screws to make fine adjustments to level
the bed support if required.
Tip:
If necessary, the jacks on the back wheels of the bed support lifting device
can be used to level the lifting device.
9
Make sure that the center of the bed support aligns with the center of the
distributor plate. Stop winching when there is contact with the distributor
O-ring and the bed support fasteners have entered the corresponding holes
in the adapter.
Note:
Make sure that the thin outer O-ring does not become dislodged, deformed
or trapped when winching up the bed support.
256 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.3 Assembling the adapter
ActionStep
Attach the plastic and stainless steel washers and fastening nuts to the
threads of the bed support fasteners, with the plastic washers closest to the
backing plate. Finger-tighten the nuts onto the fasteners. Make sure that
there is no gap between the assembly and the backing plate all the way
around the circumference of the column.
10
Pull out the spring loaded pin on the distributor fitting, and then lower the
bed support lifting device until it is well clear of the adapter, using the rear
side winch. Carefully move the bed support lifting device away from the
column.
11
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8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.3 Assembling the adapter
ActionStep
Wet the threads of the bed support tool, and then use the reverse end of
the bed support tool to center the bed support. With the bed support tool in
place, use a torque wrench to tighten all of the bed support fastening nuts
crosswise, in increments to a torque of 30 Nm, then remove the bed support
tool.
12
NOTICE
Make sure that the bed support is raised evenly against
the distributor as the bed support fastening nuts are
tightened. Perform a final check around the entire cir-
cumference of the column after the final tension has
been reached to make sure that there are no gaps and
that the bed support is fully in contact with the distrib-
utor around the entire column.
258 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.3 Assembling the adapter
ActionStep
Make sure that surfaces are wetted with 20% ethanol, and then insert the
bed support screw into the center of the bed support and carefully finger
tighten it, making sure that the threads are not crossed. Finally tighten the
bed support screw firmly using the bed support tool, making sure that the
bed support is secured in place by the bed support screw.
13
NOTICE
Be careful not to damage the bed support or the bed
support screw when using the bed support tool.
Refit the rinse nozzle
If there is little clearance space available to maneuver the rinse nozzle, pres-
surized air can be used to blow the nozzle down into its open (lowest) position.
Tip:
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 259
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.3 Assembling the adapter
ActionStep
If the nozzle has been disassembled, assemble the rinse nozzle in accordance
with the instructions in Section 8.2 Rinse nozzle maintenance, on page 207.
Tip:
To improve the amount of clearance space above the rinse nozzle when refit-
ting the nozzle into the column, do not fit the mobile phase inlet/outlet con-
nection tube until after the nozzle has been maneuvered into position and
lowered into the center of the adapter.
1
then…If…
lubricate the top inlet spacer and corresponding
additional O-ring with 20% ethanol, and fit the
top inlet spacer and O-ring onto the nozzle body
before proceeding to the next step.
the column is an
AxiChrom 1200 col-
umn,
Carefully align the nozzle with the corresponding guide positions in the
backing plate, and then carefully lower the rinse nozzle into the column.
2
260 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.3 Assembling the adapter
ActionStep
Fit washers onto the four outer nozzle bolts, and then insert the bolts into
the nozzle and finger tighten.
3
Tighten the nozzle bolts, crosswise and in steps, using a calibrated torque
wrench, to a torque of 5 Nm.
4
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 261
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.3 Assembling the adapter
Refit the bed support
NOTICE
Clean O-ring grooves and lubricate all O-rings thoroughly with 20%
ethanol before fitting O-rings into place.
ActionStep
Lubricate and then refit the O-ring on the distributor plate.1
Lubricate and refit the two small O-rings into their respective O-ring groove
in the center of the the bed support, then lubricate and fit the large bed
support O-ring into the bed support O-ring groove. Make sure that the large
O-ring is properly seated in its groove.
Note:
There are two central bed support O-rings, one on each side of the center of
the bed support. Both of these O-rings cannot be inserted at the same time
when the distributor plate is attached to the lifting device. The O-ring that is
situated on the lifting device side of the distributor plate can be attached before
or after the lifting device is used.
2
Attach the bed support to the bed support lifting device as described in Re-
moving the bed support, on page 230.
3
Carefully remove any old PTFE tape from the bed support fasteners, and
then wrap the threads of all of the bed support fasteners with new PTFE
tape.
4
264 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.4 Assembling the bottom assembly
ActionStep
Move the bed support lifting device into position above the distributor plate
so that the middle of the tri-arm and bed support is directly over the middle
of the distributor plate.
5
Take care not to bump the bed support into the column stand.
Note:
To correctly align the tri-arm screw fittings of the bed support lifting device
with the corresponding threaded holes in the bed support or distributor plate
of the bottom columnassembly, the bed support lifting devicemust bemoved
into position from the side of the column as shown in the following illustration.
Make sure that the bed support fasteners are aligned with the corresponding
holes in the distributor plate. Make sure that the O-ring on the distributor is
wetted with 20% ethanol.
6
Use the rear winch to lower the bed support until it rests on the bottom dis-
tributor plate. Make sure that the bed support is flat against the distributor
plate and is not tilted.
Tip:
If necessary, the jacks on the back wheels of the bed support lifting device
can be used to level the lifting device.
7
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 265
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.4 Assembling the bottom assembly
ActionStep
Loosen the counter nuts of the tri-arm threaded screws, and unscrew the
tri-arm threaded screw fastenings from the bed support.
8
Tighten the counter nuts again and make sure that the tri-arm threaded
screw fittings are securely attached to the tri-arms again and are clear of
the bed support.
9
Use the rear side winch to lift the tri-arm until it is well clear of the bed sup-
port. Carefully move the bed support lifting device away from the column.
10
Check that the bed support is still flat against the distributor plate, then fit
the washers and bed support fastening nuts to the bed support fasteners
protruding from the underside of the backing plate. Finger tighten the fas-
tening nuts.
11
Insert the threaded end of the bed support tool into the center of the backing
plate and screw into place.
12
With the bed support tool in place, use a torque wrench to tighten all of the
bed support fastening nuts crosswise, in increments to a torque of 15 Nm.
Note:
The bed support fastening nuts can loosen when the tube is fastened into
place. Check torque tensions and retighten to 15 Nm as required.
13
Unscrew and remove the bed support tool from the center of the bed support.14
Lubricate the bed support screw O-ring and the bed support screw with
20% ethanol, and then fit the O-ring onto the bed support screw.
15
266 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.4 Assembling the bottom assembly
ActionStep
Make sure that surfaces are wetted with 20% ethanol, and then insert the
bed support screw into the center of the bed support and carefully finger
tighten it, making sure that the threads are not crossed. Finally tighten the
bed support screw firmly using the bed support tool, making sure that the
bed support is secured in place by the bed support screw.
16
NOTICE
Be careful not to damage the bed support or the bed
support screw when using the bed support tool.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 267
8 Maintenance of 1200-1600 columns
8.4 Maintaining columns with stainless steel bed supports
8.4.4 Assembling the bottom assembly
8.5.1 Disassembling the bottom assembly
Removing the bed support
ActionStep
Make sure the media valve is open. The media valve is open when the piston
in the center of the bed support is lowered. If the media valve is closed, it
can be opened in the MANUAL CONTROL wizard in AxiChrom Master.
1
Unscrew the bed support nuts (with screws underneath that sometimes also
come off when the nuts are removed).
Note:
If only the nut comes off, leave the screw in place but make sure to lift the
bed support carefully and straight upwards in the following step, so that it is
not damaged by the screws.
2
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8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.1 Disassembling the bottom assembly
ActionStep
Use the bed support tool to remove the bed support screw in the center.3
NOTICE
Be careful not to slip with the bed support tool since
this may damage the bed support screw and/or the
bed support.
NOTICE
Be careful not to damage the threads when removing
the bed support screw.
Remove the bed support by hand, lifting it straight upwards and making
sure that any remaining screws do not damage the bed support. If necessary,
insert the O-ring removal tool between the edge of the bed support and the
O-ring and gently lever the bed support out of its seating.
4
Remove the inner and outer O-rings from the bed support screw. Clean the
O-rings and the bed support screw with 20% ethanol. Replace with new O-
rings if necessary.
5
Remove the O-ring from the distributor plate. Clean the distributor plate, O-
ring groove and O-ring with 20% ethanol. Replace with a new O-ring if nec-
essary.
6
272 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.1 Disassembling the bottom assembly
8.5.2 Disassembling the adapter
It is recommended that at least two peoplework togetherwhen disassembling
the adapter.
Note:
Remove the rinse nozzle
Remove the rinse nozzle using the same procedure as described for columns with
stainless steel bed supports, see Remove the rinse nozzle, on page 238.
Remove the distributor and bed
support assembly
NOTICE
During all maintenance on the adapter and rinse nozzle make sure
that the bottom bed support and the bottom distributor are pro-
tected, for example by covering them with a plastic film and a bed
protection mat.
274 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.2 Disassembling the adapter
ActionStep
Use the bed support tool to remove the bed support screw from the center
of the bed support. Hold the bed support tool vertically when removing the
bed support screw.
1
NOTICE
Be careful not to slip with the bed support tool since
this may damage the bed support screw.
NOTICE
Be careful not to damage the threads when removing
the bed support screw.
Remove the O-ring on the bed support screw. Clean the O-ring with 20%
ethanol or replace with a new O-ring if necessary.
2
Remove the four flushing connectors according to the instruction in Sec-
tion 6.7 Changing flushing connector O-rings, on page158. Clean the O-rings
of the flushing connectors with 20% ethanol. Replace with new O-rings if
necessary.
3
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 275
8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.2 Disassembling the adapter
ActionStep
Remove the four bolts underneath the rinse nozzle.4
Remove the bolts on the top of the distributor plate. The number of bolts
varies with columns size.
5
Wet the threads of the distributor plate fitting, and then fasten the distributor
plate fitting through the bed support and into the distributor plate center.
6
276 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.2 Disassembling the adapter
ActionStep
Make sure that the bed support lifting device tri-arms are equipped with the
lower clamps. If the tri-arms are not equipped with the lower clamps, install
the lower clamps as shown in the illustration.
7
Loosen the stabilizing screws in the center of the tri-arms of the bed support
lifting device. This will make it easier to correctly fasten the tri-arms to the
bed support.
8
Move the bed support lifting device into position below the bed support,
moving the lifting device in from the side of the column, as shown in the il-
lustration, so that the middle of the tri-arm is directly under the middle of
the bed support and the tri-arm ends are under the outer rim of the bed
support.
9
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 277
8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.2 Disassembling the adapter
ActionStep
Fasten the stabilizing screws at the center of the tri-arms.12
Slowly lower the tri-arm, while making sure the distributor plate separates
from the adapter.
13
NOTICE
The lifting device must be lowered far enough to make
sure that the fasteners on the top of the bed support
cannot scrape the polypropylene surface of the distrib-
utor.
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 279
8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.2 Disassembling the adapter
ActionStep
Move the bed support lifting device carefully and slowly, taking care to avoid
contact between the bed support lifting device and the column stand. Move
the bed support lifting device only far enough to allow access to remove the
scraper seals, O-rings and distributor ring.
Note:
The upper tri-arm clamps cannot be used to hold the assembly in place until
the scraper seals, O-rings and distributor ring have been removed from the
distributor. Do not move the lifting device any further than necessary until
the upper clamps are fitted after removal of the scraper seals, O-rings and
distributor ring.
14
Disassemble the adapter
distributor and bed support
ActionStep
Remove the upper scraper seal and O-ring. Inspect the scraper seal and O-
ring and clean with 20% ethanol or replace with new components if neces-
sary.
1
280 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.2 Disassembling the adapter
ActionStep
Use a hex key to remove the screws holding the distributor ring, and then
remove the distributor ring from the distributor.
2
Remove the lower scraper seal and the two O-rings. Clean the seal and O-
rings with 20% ethanol or replace with new components if necessary.
3
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 281
8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.2 Disassembling the adapter
ActionStep
Fasten the distributor and bed support assembly to the bed support lifting
device by fitting the upper clamps onto the tri-arms, and then use the side
winch of the bed support lifting device to rotate the tri-arms through 180
degrees.
4
NOTICE
Make sure that the tri-arm clamps and center clamp
are securely fastened, so that the bed support and/or
distributor cannot fall when the arms of the lifting de-
vice are rotated.
1 2 3
FunctionPart
Tri-arm threaded screw fitted with upper and lower clamps1
Bed support nut2
Snap ring3
Move the bed support lifting device so that the distributor assembly is posi-
tioned above a clean surface, such as a stable bench top or work surface
covered with suitable protective material.
5
Lower the tri-arm and place the bed support and distributor assembly onto
the supporting surface, allowing the assembly to rest on the fasteners or on
the distributor itself.
6
282 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.2 Disassembling the adapter
ActionStep
Remove the upper clamps, release the center fitting spring lock, and then
winch up the tri-arm and move the bed support lifting device aside.
7
Remove the bed support nuts.8
Remove the snap ring using the O-ring removal tool.9
Remove the central distributor plate fitting, and then carefully lift the bed
support away from the distributor.
10
Remove the gaskets from around the studs in the pockets of the distributor.
Clean and inspect the gaskets and renew these if necessary. Check that all
of the studs are firmly in position in their respective pockets.
11
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 283
8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.2 Disassembling the adapter
8.5.3 Assembling the adapter
At least two persons are needed when assembling the adapter.Note:
Reassemble the bed support
ActionStep
Fit the gaskets into the recessed pockets around the studs in the distributor.1
Place the new bed support on the distributor, with the recessed screw-hole
side of the bed support facing upwards.
2
Refit the bed support nuts (replace where necessary). The bed support nuts
should be inserted so that they all just enter the extrusion in the sinter. Do
not tighten the nuts.
3
Tighten the bed support nuts to a torque of 1.8 Nm.4
284 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.3 Assembling the adapter
ActionStep
Refit the snap ring. Tap gently with the plastic mallet if necessary. Use the
groove on the mallet head to lever the snap ring over the edge of the bed
support if the fit is tight.
Note:
Check that the snap ring is correctly and evenly mounted around the whole
circumference of the bed support.
5
The illustration shows a cross-section of a snap-ring mounted correctly and
two images of a snap-ring mounted incorrectly.
A
BC
D
FunctionPart
Snap ringA
Scraper sealB
DistributorC
Bed supportD
Fasten the distributor plate fitting into center of the bed support, and then
move the bed support lifting device into position above the bed support so
that the middle of the tri-arm is directly above the middle of the bed support
and the tri-arm ends are above the outer rim of the bed support.
6
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 285
8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.3 Assembling the adapter
ActionStep
Make sure that the bolt holes, including flushing ports, in the adapter backing
plate are fully aligned with the corresponding holes in the distributor plate.
3
Use the rear winch to gently lift the tri-arm upwards. Make sure that the bed
support is in level when raised. Use the stabilizing screws to adjust the level.
4
Make sure that the center of the bed support aligns with the center of the
adapter backing plate. Stop winching when the fastener is well through the
adapter.
Tip:
If necessary, the jacks on the back wheels of the bed support lifting device
can be used to level the lifting device.
Attach the bed support fastening nuts and plastic and stainless steel
washers, with the plastic washers closest to the backing plate. Finger tighten
the nuts onto the fasteners. Make sure that there is no gap between the
assembly and the backing plate.
5
288 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.3 Assembling the adapter
ActionStep
Refit the four bolts into the bolt positions underneath the adapter rinse
nozzle and finger tighten these bolts, making sure that the distributor is
correctly positioned and centered.
6
Pull out the spring loaded pin on the distributor fitting and lower the lifting
device using the rear side winch, and then slowly remove the bed support
lifting device.
7
Refit the bolts on top of the distributor plate. Fit both stainless steel and
plastic washers on each screw, with the plastic washer closest to the distrib-
utor plate. Tighten the bolts to 15 Nm.
8
The following image illustrates the bolts holding the distributor plate. The
number of bolts will vary depending on column size. A: Stainless steel
washer. B: Plastic washer.
A
B
Tighten all of the bed support fastening nuts, crosswise, to a torque of 6 Nm.9
Remove the distributor fitting from the distributor plate.10
Tighten the four central bolts, the bolt positions underneath the adapter
rinse nozzle, to a torque of 11 Nm.
11
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 289
8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.3 Assembling the adapter
ActionStep
Refit the four flushing connectors according to the assembly instructions in
Section 6.7 Changing flushing connector O-rings, on page 158.
12
Refit the O-ring on the bed support screw, then wet the O-ring and screw
with 20% ethanol.
13
Spray the surfaces at the center of the bed support with 20% ethanol, then
refit the bed support screw into the center of the bed support. Carefully start
to tighten the bed support screw by hand making sure that the threads are
not crossed.
14
Tighten the bed support screw firmly using the bed support tool. Make sure
the bed support is secured by the bed support screw.
NOTICE
Be careful not to damage the bed support or the bed
support screw when using the bed support tool.
290 AxiChrom 300-1600 columns Operating Instructions 29065430 AD
8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.3 Assembling the adapter
ActionStep
Refit the bed support nuts (replace where necessary). The bed support nuts
should be inserted so that they all just enter the extrusion in the sinter. Do
not tighten the nuts.
4
Wet the bed support screw, and then refit the bed support screw. Carefully
start to tighten the bed support screw by hand, making sure that the threads
match. Finally tighten the bed support screw until it reaches a firm stop.
5
NOTICE
Be careful not to damage the bed support or the bed
support screw when using the bed support tool.
Tighten the bed support nuts to a torque of 1.8 Nm.6
AxiChrom 300-1600 columns Operating Instructions 29065430 AD 293
8 Maintenance of 1200-1600 columns
8.5 Maintaining columns with plastic bed supports
8.5.4 Assembling the bottom assembly
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